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Literature summary extracted from

  • Ambrus, A.; Torocsik, B.; Adam-Vizi, V.
    Periplasmic cold expression and one-step purification of human dihydrolipoamide dehydrogenase (2009), Protein Expr. Purif., 63, 50-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expressed in Escherichia coli JM83 cells Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 55000
-
 2 * 55000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Homo sapiens P09622
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 Strep-Tactin Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 lipoic acid + NADH
-
 Homo sapiens dihydrolipoic acid + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.4 homodimer 2 * 55000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Homo sapiens
1.8.1.4 E3 subunit of the alpha-ketoglutarate dehydrogenase complex
-
 Homo sapiens
1.8.1.4 LADH
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD
-
 Homo sapiens
1.8.1.4 NADH
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.8.1.4 physiological function dihydrolipoamide dehydrogenase is a FAD-linked subunit of 2-oxooglutarate, pyruvate and branched-chain amino acid dehydrogenases and the glycine cleavage system, transfering electrons from the dihydrolipoic acid prosthetic group to the NAD+ cofactor via its FAD center Homo sapiens