Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, Z.; Xu, J.; He, Y.; Ouyang, L.; Liu, Y.; Imanaka, T.
    Efficient production of (R)-(-)-mandelic acid with highly substrate/product tolerant and enantioselective nitrilase of recombinant Alcaligenes sp. (2010), Process Biochem., 45, 887-891.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli JM109 cells Alcaligenes sp.

General Stability

EC Number General Stability Organism
3.5.5.1 the recombinant nitrilase (from Escherichia coli strain JM109/pNLE) is quite stable, the residual activity after 10 cycles of reaction is still over 40% of the initial activity Alcaligenes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 21.8
-
 mandelonitrile at 30°C and pH 8.0 Alcaligenes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 38500
-
 x * 38500, calculated from amino acid sequence Alcaligenes sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Alcaligenes sp. C5IIS9
-
-
3.5.5.1 Alcaligenes sp. ECU0401 C5IIS9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
 Alcaligenes sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 25.6
-
 purified enzyme, using mandelonitrile as substrate, at 30°C and pH 8.0 Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 mandelonitrile + 2 H2O
-
 Alcaligenes sp. (R)-mandelic acid + NH3
-
 ?
3.5.5.1 mandelonitrile + 2 H2O
-
 Alcaligenes sp. ECU0401 (R)-mandelic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 ? x * 38500, calculated from amino acid sequence Alcaligenes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 45
-
-
 Alcaligenes sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 35 60
-
 Alcaligenes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 30 50 the enzyme is quite stable at 30°C, with half-life of 63 h, the enzyme is labile at higher temperatures as revealed by half-lives of 35 h and 22 h at 40°C and 50°C, respectively Alcaligenes sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.1 175
-
 mandelonitrile at 30°C and pH 8.0 Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 8
-
-
 Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 7 8.6 the nitrilase activity was low at pH less 7.0 or pH above 8.6 Alcaligenes sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.5.1 8
-
 mandelonitrile at 30°C and pH 8.0 Alcaligenes sp.