Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhi, Q.; Kong, P.; Zang, J.; Cui, Y.; Li, S.; Li, P.; Yi, W.; Wang, Y.; Chen, A.; Hu, C.
    Biochemical and molecular characterization of a novel high activity creatine amidinohydrolase from Arthrobacter nicotianae strain 02181 (2009), Process Biochem., 44, 460-465.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.3 N3- 1 mM, 104% of initial activity Glutamicibacter nicotianae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.3
-
 Glutamicibacter nicotianae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.3 Cu2+ 1 mM, no residual activity Glutamicibacter nicotianae
3.5.3.3 Fe3+ 1 mM, 2.2% residual activity Glutamicibacter nicotianae
3.5.3.3 Hg2+ 1 mM, 9.8% residual activity Glutamicibacter nicotianae
3.5.3.3 sodium dodecylsulfate 5 g/l, no residual activity Glutamicibacter nicotianae
3.5.3.3 Zn2+ 1 mM, 44% residual activity Glutamicibacter nicotianae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.3 46
-
 Creatine pH 7.0, 37°C Glutamicibacter nicotianae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.3 Ca2+ 1 mM, 104% of initial activity Glutamicibacter nicotianae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.3 46400
-
 2 * 46400, SDS-PAGE Glutamicibacter nicotianae
3.5.3.3 90000
-
 PAGE Glutamicibacter nicotianae

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Glutamicibacter nicotianae A7LCN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.3
-
 Glutamicibacter nicotianae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.3 124.4
-
 pH 7.0, 37°C Glutamicibacter nicotianae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.3 creatine + H2O
-
 Glutamicibacter nicotianae sarcosine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.3 dimer 2 * 46400, SDS-PAGE Glutamicibacter nicotianae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.3 37
-
-
 Glutamicibacter nicotianae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.3 25 45
-
 Glutamicibacter nicotianae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.3 45
-
 stable below Glutamicibacter nicotianae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.3 7
-
-
 Glutamicibacter nicotianae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.3 6 8
-
 Glutamicibacter nicotianae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.3 6 9
-
 Glutamicibacter nicotianae