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Literature summary extracted from

  • Juarez, O.; Morgan, J.E.; Nilges, M.J.; Barquera, B.
    Energy transducing redox steps of the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae (2010), Proc. Natl. Acad. Sci. USA, 107, 12505-12510.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
7.2.1.1 C76A mutant of subunit NqrF which lacks the 2Fe-2S-center cofactor and shows a 90% decrease in menadione reductase activity Vibrio cholerae serotype O1
7.2.1.1 D346A the redox reaction in this mutant is significantly inhibited and the Na+ sensitivity of CoQ reduction is completely abolished, while the apparent affinity for Na+ is essentially unchanged. In D346A, electron transfer between FMN from subunit NqrB and riboflavin is inhibited Vibrio cholerae serotype O1
7.2.1.1 D397A the reduction of subunit NqrB mutant D397A by NADH is very similar to the reduction of wild type enzyme but does not show sensitivity to Na+, and the rate of the stable neutral riboflavin radical to riboflavin-H2 is decreased 3fold. In NqrB-D397A, the reaction takes place in two steps: rapid oxidation of riboflavin and the two FMNs, followed by oxidation of FAD, at a 16fold lower rate Vibrio cholerae serotype O1
7.2.1.1 T225Y the mutant lacks FMN from subunit NqrC and is unable to produce an electrochemical potential under either partial or multiple turnover conditions Vibrio cholerae serotype O1
7.2.1.1 T236Y the mutant lacks FMN from subunit NqrB and is unable to produce an electrochemical potential under either partial or multiple turnover conditions Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
 NAD+ + ubiquinol + n Na+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.1 Vibrio cholerae serotype O1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.1.1 Na+-NQR
-
 Vibrio cholerae serotype O1
7.2.1.1 Na+-pumping NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1
7.2.1.1 sodium pumping NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.1 FAD contains one noncovalently bound FAD Vibrio cholerae serotype O1
7.2.1.1 FMN contains two FMNs covalently bound to the NqrB and NqrC subunits Vibrio cholerae serotype O1
7.2.1.1 NADH
-
 Vibrio cholerae serotype O1
7.2.1.1 riboflavin contains one noncovalently bound riboflavin Vibrio cholerae serotype O1
7.2.1.1 [2Fe-2S]-center contains one 2Fe-2S-center Vibrio cholerae serotype O1

General Information

EC Number General Information Comment Organism
7.2.1.1 metabolism Na+-NQR couples the free energy of electron transfer reactions to electrogenic pumping of sodium across the cell membrane. Na+ uptake by Na+-NQR is a nonelectrogenic process that occurs upon electron transfer from the 2Fe-2S-center to FMN of subunit NqrC, while the electrogenic transport of Na+ across the membrane, and likely release into solution, occurs upon electron transfer from FMN of subunit NqrB to riboflavin Vibrio cholerae serotype O1