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Literature summary extracted from

  • Erb, T.J.; Brecht, V.; Fuchs, G.; Muller, M.; Alber, B.E.
    Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase (2009), Proc. Natl. Acad. Sci. USA, 106, 8871-8876.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.85 0.2
-
 CO2 at pH 7.8, temperature not specified in the publication Cereibacter sphaeroides
1.3.1.85 0.2
-
 (E)-crotonyl-CoA for reduction, pH and temperature not specified in the publication Cereibacter sphaeroides
1.3.1.85 0.4
-
 (E)-crotonyl-CoA for reductive carboxylation, pH and temperature not specified in the publication Cereibacter sphaeroides
1.3.1.85 0.5
-
 acryloyl-CoA pH and temperature not specified in the publication Cereibacter sphaeroides
1.3.1.85 0.7
-
 NADPH pH and temperature not specified in the publication Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.85 47000
-
 2 * 47000, gel filtration Cereibacter sphaeroides
1.3.1.85 105000
-
 gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.85 (E)-crotonyl-CoA + CO2 + NADPH + H+ Cereibacter sphaeroides 100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction (2S)-ethylmalonyl-CoA + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.85 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.85
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.85 (E)-crotonyl-CoA + CO2 + NADPH + H+ 100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction Cereibacter sphaeroides (2S)-ethylmalonyl-CoA + NADP+
-
 r
1.3.1.85 (E)-crotonyl-CoA + NADPH + H+ 10% of maximal activity (compared with vmax of crotonyl-CoA carboxylation) Cereibacter sphaeroides butyryl-CoA + NADP+
-
 ?
1.3.1.85 acryloyl-CoA + CO2 + NADPH + H+ acryloyl-CoA is accepted as an alternative substrate analogue by the enzyme with 40% relative activity (compared with vmax of crotonyl-CoA carboxylation) Cereibacter sphaeroides methylmalonyl-CoA + NADP+
-
 ?
1.3.1.85 additional information does not reduce crotonyl-N-acetylcysteamine, methacryloyl-CoA, 6-hydroxycylohex-1-ene-1-carboxyl-CoA, cyclo-hexa-1,5-diene-1-carboxyl-CoA, acetoacetyl-CoA, propionyl-CoA, (R)-3-hydroxybutyryl-CoA, (S)-3-hydroxybutyryl-CoA (less than 1% specific activity) Cereibacter sphaeroides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.85 homodimer 2 * 47000, gel filtration Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
1.3.1.85 CCR
-
 Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.85 7.5 8
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.85 NADPH
-
 Cereibacter sphaeroides