EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.87 | expression of structure-based mutants of ADAMTS13-MDTCS residues 75-685 fragment with a C-terminal tobacco etch virus proteinase cleavage site followed by tandem His-tag sequences in CHO Lec 3.2.8.1 cells. Transient expression of His-tagged mutant ADAMTS13-MDTCS domains using a cytomegalovirus promoter-driven expression vector in HeLa cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.24.87 | purified recombinant detagged ADAMTS13-DTCS, residues 287-685, an non-catalytic von Willebrand factor-exosite-containing ADAMTS13 fragment, sitting drop vapor diffusion method, mixing of 500 nl of protein solution and 500 nl reservoir solution containing 26% w/v PEG1500, 100 mM MES, pH 6.0, supplemented with 200 nl of 40% w/w pentaerythritol ethoxylate, equilibration for several days at 20°C. Os-derivative crystals by soaking native crystals in reservoir solution supplemented with 1 mM OsCl3 and 20% glycerol for several hours, X-ray diffraction structure determination and analysis at 2.6 A and 2.8 A resolution resulting in two crystal structures | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.87 | additional information | generation of structure-based mutants of ADAMTS13-MDTCS residues 75685 fragment. The MDTCS domains are conserved among ADAMTS family proteins | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.87 | extracellular | - |
Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.87 | Ca2+ | required | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.87 | additional information | Homo sapiens | MDTCS domain modelling and substrate recognition mode analysis, overview | ? | - |
? | |
3.4.24.87 | von Willebrand factor + H2O | Homo sapiens | specific for | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.87 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.87 | recombinant structure-based mutants of ADAMTS13-MDTCS residues 75-685 fragment with a C-terminal tobacco etch virus proteinase cleavage site followed by tandem His-tag sequences from CHO Lec 3.2.8.1 cells by nickel affinity chromatography and gel filtration | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.87 | plasma | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.87 | FRETS-VWF73 peptide + H2O | fluorogenic von Willebrand factor-derived peptide substrate | Homo sapiens | ? | - |
? | |
3.4.24.87 | additional information | MDTCS domain modelling and substrate recognition mode analysis, overview | Homo sapiens | ? | - |
? | |
3.4.24.87 | von Willebrand factor + H2O | specific for | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.87 | More | ADAMTS13-DTCS, residues 287-685, an exosite-containing ADAMTS13 fragment, structures reveal folding similarities between the disintegrin-like domain and the N-terminal portion of the cysteine-rich domain. The spacer domain forms a globular functional unit with a 10-stranded-sandwich fold that has multiple interaction sites with the cysteine-rich domain. The MDTCS domains are conserved among ADAMTS family proteins | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.87 | ADAMTS13 | ADAMTS13 belongs to the ADAMTS or a disintegrin-like and metalloproteinase with thrombospondin type-1 motif family | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.87 | 7.4 | - |
assay at | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.24.87 | physiological function | ADAMTS13 specifically cleaves plasma von Willebrand factor and thereby controls von Willebrand factor-mediated platelet thrombus formation. Severe deficiencies in ADAMTS13 can cause life-threatening thrombotic thrombocytopenic purpura | Homo sapiens |