Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Akiyama, M.; Takeda, S.; Kokame, K.; Takagi, J.; Miyata, T.
    Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor (2009), Proc. Natl. Acad. Sci. USA, 106, 19274-19279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.87 expression of structure-based mutants of ADAMTS13-MDTCS residues 75-685 fragment with a C-terminal tobacco etch virus proteinase cleavage site followed by tandem His-tag sequences in CHO Lec 3.2.8.1 cells. Transient expression of His-tagged mutant ADAMTS13-MDTCS domains using a cytomegalovirus promoter-driven expression vector in HeLa cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.87 purified recombinant detagged ADAMTS13-DTCS, residues 287-685, an non-catalytic von Willebrand factor-exosite-containing ADAMTS13 fragment, sitting drop vapor diffusion method, mixing of 500 nl of protein solution and 500 nl reservoir solution containing 26% w/v PEG1500, 100 mM MES, pH 6.0, supplemented with 200 nl of 40% w/w pentaerythritol ethoxylate, equilibration for several days at 20°C. Os-derivative crystals by soaking native crystals in reservoir solution supplemented with 1 mM OsCl3 and 20% glycerol for several hours, X-ray diffraction structure determination and analysis at 2.6 A and 2.8 A resolution resulting in two crystal structures Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.87 additional information generation of structure-based mutants of ADAMTS13-MDTCS residues 75–685 fragment. The MDTCS domains are conserved among ADAMTS family proteins Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.87 extracellular
-
Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.87 Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.87 additional information Homo sapiens MDTCS domain modelling and substrate recognition mode analysis, overview ?
-
?
3.4.24.87 von Willebrand factor + H2O Homo sapiens specific for ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.87 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.87 recombinant structure-based mutants of ADAMTS13-MDTCS residues 75-685 fragment with a C-terminal tobacco etch virus proteinase cleavage site followed by tandem His-tag sequences from CHO Lec 3.2.8.1 cells by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.87 plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.87 FRETS-VWF73 peptide + H2O fluorogenic von Willebrand factor-derived peptide substrate Homo sapiens ?
-
?
3.4.24.87 additional information MDTCS domain modelling and substrate recognition mode analysis, overview Homo sapiens ?
-
?
3.4.24.87 von Willebrand factor + H2O specific for Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.87 More ADAMTS13-DTCS, residues 287-685, an exosite-containing ADAMTS13 fragment, structures reveal folding similarities between the disintegrin-like domain and the N-terminal portion of the cysteine-rich domain. The spacer domain forms a globular functional unit with a 10-stranded-sandwich fold that has multiple interaction sites with the cysteine-rich domain. The MDTCS domains are conserved among ADAMTS family proteins Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.87 ADAMTS13 ADAMTS13 belongs to the ADAMTS or a disintegrin-like and metalloproteinase with thrombospondin type-1 motif family Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.87 7.4
-
assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.24.87 physiological function ADAMTS13 specifically cleaves plasma von Willebrand factor and thereby controls von Willebrand factor-mediated platelet thrombus formation. Severe deficiencies in ADAMTS13 can cause life-threatening thrombotic thrombocytopenic purpura Homo sapiens