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Literature summary extracted from

  • Close, D.M.; Patterson, S.S.; Ripp, S.; Baek, S.J.; Sanseverino, J.; Sayler, G.S.
    Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line (2010), PLoS ONE, 5, e12441.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 the bacterial luciferase lux gene cassette consists of five genes, luxCDABE. The lux operon is re-synthesized through a process of multibicistronic, codon-optimization to demonstrate self-directed bioluminescence emission in a mammalian HEK-293 cell line in vitro and in vivo, overview. To overcome the limitations by FMNH2 supply, co-expression of a constitutively expressed flavin reductase gene frp from Vibrio harveyi is performed leading to a 151fold increased increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes Vibrio harveyi

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.3 additional information codon optimization of the luxCDE and frp genes, e.g. adaptation of the bacterial protein to mammalian temperature of 37°C, detailed overview Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 an aldehyde + FMNH2 + O2 Vibrio harveyi
-
 a carboxylate + FMN + H2O + hnu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 an aldehyde + FMNH2 + O2
-
 Vibrio harveyi a carboxylate + FMN + H2O + hnu
-
 ?
1.14.14.3 an aldehyde + FMNH2 + O2 a long chain aliphatic aldehyde as substrate Vibrio harveyi a carboxylate + FMN + H2O + hnu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 luciferase
-
 Vibrio harveyi

General Information

EC Number General Information Comment Organism
1.14.14.3 physiological function the luxCDABE encoded protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations Vibrio harveyi