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Literature summary extracted from

  • Han, Y.; Vimolmangkang, S.; Soria-Guerra, R.E.; Rosales-Mendoza, S.; Zheng, D.; Lygin, A.V.; Korban, S.S.
    Ectopic expression of apple F3'H genes contributes to anthocyanin accumulation in the Arabidopsis tt7 mutant grown under nitrogen stress (2010), Plant Physiol., 153, 806-820.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.82 gene MdF3'HI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum Malus domestica
1.14.14.82 gene MdF3'HIIa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum Malus domestica
1.14.14.82 gene MdF3'HIIb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum Malus domestica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.82 microsome
-
 Malus domestica
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 Malus domestica
-
 dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2 Malus domestica
-
 quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Malus domestica
-
 eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.82 Malus domestica C5IGQ3 gene MdF3'HI; gene MdF3'HI
-
1.14.14.82 Malus domestica C5IGQ4 gene MdF3'HIIa; gene MdF3'HIIa
-
1.14.14.82 Malus domestica C5IGQ5 gene MdF3'HIIb; gene MdF3'HIIb
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.82 fruit throughout the development of fruits, the transcriptional levels of MdF3'H genes along with other anthocyanin biosynthesis genes are higher in the red-skinned cv. Red Delicious than that in the yellow-skinned cv. Golden Delicious. Patterns of MdF3'H gene expression correspond to accumulation patterns of flavonoids in apple fruit Malus domestica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Malus domestica dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Malus domestica quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Malus domestica eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.82 F3'H
-
 Malus domestica
1.14.14.82 flavonoid 3'-hydroxylase
-
 Malus domestica
1.14.14.82 More F3'H is a microsomal cytochrome P450-dependent monooxygenase Malus domestica

Expression

EC Number Organism Comment Expression
1.14.14.82 Malus domestica ectopic expression of apple F3'H genes contributes to anthocyanin accumulation in the Arabidopsis thaliana transparent testa7-1 mutant tt7 grown under nitrogen stress, overview. Transgenic Arabidopsis tt7 seedlings xpressing apple F3'H regain red color pigmentation and significantly accumulate both 4'-hydroxylated pelargonidin and 3',4'-hydroxylated cyanidin additional information

General Information

EC Number General Information Comment Organism
1.14.14.82 evolution duplication of F3'H genes in plants, overview Malus domestica
1.14.14.82 metabolism MdF3'H genes are coordinately expressed with other genes in the anthocyanin biosynthetic pathway in apple Malus domestica
1.14.14.82 physiological function the enzyme introduces a hydroxyl group at 3' position of the B-ring of the flavonoid molecule leading to the formation of 3',4'-hydroxylated flavonoids Malus domestica