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Literature summary extracted from

  • Liu, C.J.; Deavours, B.E.; Richard, S.B.; Ferrer, J.L.
    Blount, J.W.; Huhman, D.; Dixon, R.A.; Noel, J.P.: Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses (2006), Plant Cell, 18, 3656-3669.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.212 expression in Escherichia coli Medicago truncatula
2.1.1.270 expression in Escherichia coli Medicago truncatula

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.212 crystallized from a polyethylene glycol precipitant containing 2.5 mM of the reaction product S-adenosyl-L-homocysteine, 2.5 A resolution Medicago truncatula
2.1.1.270 crystallized from a polyethylene glycol precipitant containing 2.5 mM of the reaction product S-adenosyl-L-homocysteine, 2.5 A resolution Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.212 0.0733
-
 2,7,4'-trihydroxyisoflavanone pH 8.0, 30°C Medicago truncatula
2.1.1.212 0.0998
-
 S-adenosyl-L-methionine pH 8.0, 30°C Medicago truncatula
2.1.1.270 0.062
-
 (+)-6a-hydroxymaackiain pH 8.0, 30°C Medicago truncatula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone Medicago truncatula
-
 S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Medicago truncatula
-
 S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.212 Medicago truncatula Q29U70
-
-
2.1.1.270 Medicago truncatula Q29U70
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
-
 Medicago truncatula S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone the bifunctional enzyme also catalyzes the 3-O-methylation of (+)-6a-hydroxymaackiain, with slightly lower catalytic efficiency Medicago truncatula S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
-
 Medicago truncatula S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain the bifunctional enzyme also catalyzes the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanonewith slightly higher catalytic efficiency Medicago truncatula S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.212 HI4'OMT/HM3OMT bifunctional enzyme Medicago truncatula
2.1.1.270 HI4'OMT/HM3OMT bifunctional enzyme Medicago truncatula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.212 30
-
 assay at Medicago truncatula
2.1.1.270 30
-
 assay at Medicago truncatula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.212 8
-
 assay at Medicago truncatula
2.1.1.270 8
-
 assay at Medicago truncatula

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.212 334
-
 2,7,4'-trihydroxyisoflavanone pH 8.0, 30°C Medicago truncatula
2.1.1.270 197
-
 (+)-6a-hydroxymaackiain pH 8.0, 30°C Medicago truncatula