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Literature summary extracted from

  • Imai, T.; Niwa, M.; Ban, Y.; Hirai, M.; Oba, K.; Moriguchi, T.
    Importance of the L-galactonolactone pool for enhancing the ascorbate content revealed by L-galactonolactone dehydrogenase-overexpressing tobacco plants (2009), Plant Cell Tissue Organ Cult., 96, 105-112.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.2.3 cDNA encoding GLDH, subcloning in Escherichia coli strain HB101, and functional expression in transgenic Nicotiana tabacum leaves and roots via Agrobacterium tumefaciens LBA4404-mediated transformation under control of the CaMV 35S promoter. Despite the elevated GLDH activity, the L-ascorbate content in the leaves does not change in all lines, but increases 6-10fold in the roots of transgenic plants, overview Ipomoea batatas

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.2.3 mitochondrion
-
 Ipomoea batatas 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + cytochrome c Ipomoea batatas
-
 L-ascorbate + reduced cytochrome c
-
 ?
1.3.2.3 additional information Ipomoea batatas incubation of leaf discs in a L-galactonolactone solution leads to a rapid 2 to 3fold increase in the L-ascorbate content in both GLDH-transformed and non-transformed plants in the same manner ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.2.3 Ipomoea batatas
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.2.3 leaf
-
 Ipomoea batatas
-
1.3.2.3 root
-
 Ipomoea batatas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.2.3 0.0245 0.0513 root mitochondria of transformed transgenic tobacco lines, pH not specified in the publication, temperature not specified in the publication Ipomoea batatas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + cytochrome c
-
 Ipomoea batatas L-ascorbate + reduced cytochrome c
-
 ?
1.3.2.3 additional information incubation of leaf discs in a L-galactonolactone solution leads to a rapid 2 to 3fold increase in the L-ascorbate content in both GLDH-transformed and non-transformed plants in the same manner Ipomoea batatas ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.2.3 GalL dehydrogenase
-
 Ipomoea batatas
1.3.2.3 GLDH
-
 Ipomoea batatas
1.3.2.3 L-galactono-1,4-lactone dehydrogenase
-
 Ipomoea batatas

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.2.3 cytochrome c
-
 Ipomoea batatas

General Information

EC Number General Information Comment Organism
1.3.2.3 metabolism GLDH catalyzes the last step of L-ascorbate, AsA, biosynthesis in plants, but the upstream genes in the AsA biosynthetic pathway are responsible for enhancing the AsA content in plants, regulation, overview Ipomoea batatas