BRENDA - Enzyme Database

Arabidopsis OPT6 is an oligopeptide transporter with exceptionally broad substrate specificity

Pike, S.; Patel, A.; Stacey, G.; Gassmann, W.; Plant Cell Physiol. 50, 1923-1932 (2009) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
7.4.2.6
expression of Arabidopsis thaliana AtOPT6 in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae
Saccharomyces cerevisiae
7.4.2.6
optimization of AtOPT6 expression in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae
Arabidopsis thaliana
Organism
EC Number
Organism
UniProt
Commentary
Textmining
7.4.2.6
Arabidopsis thaliana
-
isozyme AtOPT6
-
7.4.2.6
Saccharomyces cerevisiae
-
OPT1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.4.2.6
additional information
no expression of OPTs in in the root epidermis or root hair cells
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.4.2.6
additional information
-
substrate specificity and current kinetics, overview
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
7.4.2.6
ATP + H2O + AtCLE19p12/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + AtCLE19p12/in
-
-
-
?
7.4.2.6
ATP + H2O + CLE/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA, CLE is transported by AtOPT6 with high affinity
713255
Arabidopsis thaliana
ADP + phosphate + CLE/in
-
-
-
?
7.4.2.6
ATP + H2O + GGFL/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA. GGFL-induced currents are pH-dependent
713255
Arabidopsis thaliana
ADP + phosphate + GGFL/in
-
-
-
?
7.4.2.6
ATP + H2O + GGFL/out
in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + GGFL/in
-
-
-
?
7.4.2.6
ATP + H2O + GGFM/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + GGFM/in
-
-
-
?
7.4.2.6
ATP + H2O + GSH/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA. GSH-induced currents are pH-dependent, low affinity GSH transport of AtOPT6
713255
Arabidopsis thaliana
ADP + phosphate + GSH/in
-
-
-
?
7.4.2.6
ATP + H2O + GSH/out
in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + GSH/in
-
-
-
?
7.4.2.6
ATP + H2O + GSSG/out
in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + GSSG/in
-
-
-
?
7.4.2.6
ATP + H2O + KLG/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLG/in
-
-
-
?
7.4.2.6
ATP + H2O + KLGL/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLGL/in
-
-
-
?
7.4.2.6
ATP + H2O + KLLLG/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLLLG/in
-
-
-
?
7.4.2.6
ATP + H2O + KLLLLG/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLLLLG/in
-
-
-
?
7.4.2.6
ATP + H2O + phytochelatin/out
i.e. (gamma -Glu-Cys) 2 -Gly, in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + phytochelatin/in
-
-
-
?
7.4.2.6
ATP + H2O + phytochelatin/out
i.e. (gamma -Glu-Cys) 2 -Gly, in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + phytochelatin/in
-
-
-
?
7.4.2.6
additional information
AtOPT6 displays high affinity for penta- and dodecapeptides, but low affinity for GSH, substrate specificity of AtOPT6, inward currents caused by KLLG and KLLLG application are 3 to 7fold greater than with KLG, overview
713255
Arabidopsis thaliana
?
-
-
-
?
7.4.2.6
additional information
ScOPT1 substrate specificity, overview. KLLLG, RPPGF, DRVYIHPF or AtCLE19p12 cause no inward currents in ScOPT1-expressing oocytes
713255
Saccharomyces cerevisiae
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
7.4.2.6
AtOPT6
-
Arabidopsis thaliana
7.4.2.6
glutathione transporter
-
Saccharomyces cerevisiae
7.4.2.6
oligopeptide transporter
-
Saccharomyces cerevisiae
7.4.2.6
oligopeptide transporter
-
Arabidopsis thaliana
7.4.2.6
OPT6
-
Arabidopsis thaliana
7.4.2.6
ScOPT1
-
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4.2.6
5
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.4.2.6
expression of Arabidopsis thaliana AtOPT6 in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae
Saccharomyces cerevisiae
7.4.2.6
optimization of AtOPT6 expression in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.4.2.6
additional information
no expression of OPTs in in the root epidermis or root hair cells
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.4.2.6
additional information
-
substrate specificity and current kinetics, overview
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
7.4.2.6
ATP + H2O + AtCLE19p12/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + AtCLE19p12/in
-
-
-
?
7.4.2.6
ATP + H2O + CLE/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA, CLE is transported by AtOPT6 with high affinity
713255
Arabidopsis thaliana
ADP + phosphate + CLE/in
-
-
-
?
7.4.2.6
ATP + H2O + GGFL/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA. GGFL-induced currents are pH-dependent
713255
Arabidopsis thaliana
ADP + phosphate + GGFL/in
-
-
-
?
7.4.2.6
ATP + H2O + GGFL/out
in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + GGFL/in
-
-
-
?
7.4.2.6
ATP + H2O + GGFM/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + GGFM/in
-
-
-
?
7.4.2.6
ATP + H2O + GSH/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA. GSH-induced currents are pH-dependent, low affinity GSH transport of AtOPT6
713255
Arabidopsis thaliana
ADP + phosphate + GSH/in
-
-
-
?
7.4.2.6
ATP + H2O + GSH/out
in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + GSH/in
-
-
-
?
7.4.2.6
ATP + H2O + GSSG/out
in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + GSSG/in
-
-
-
?
7.4.2.6
ATP + H2O + KLG/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLG/in
-
-
-
?
7.4.2.6
ATP + H2O + KLGL/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLGL/in
-
-
-
?
7.4.2.6
ATP + H2O + KLLLG/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLLLG/in
-
-
-
?
7.4.2.6
ATP + H2O + KLLLLG/out
in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + KLLLLG/in
-
-
-
?
7.4.2.6
ATP + H2O + phytochelatin/out
i.e. (gamma -Glu-Cys) 2 -Gly, in Xenopus laevis oocytes, injected with AtOPT6 cRNA
713255
Arabidopsis thaliana
ADP + phosphate + phytochelatin/in
-
-
-
?
7.4.2.6
ATP + H2O + phytochelatin/out
i.e. (gamma -Glu-Cys) 2 -Gly, in Xenopus laevis oocytes, injected with ScOPT1 cRNA
713255
Saccharomyces cerevisiae
ADP + phosphate + phytochelatin/in
-
-
-
?
7.4.2.6
additional information
AtOPT6 displays high affinity for penta- and dodecapeptides, but low affinity for GSH, substrate specificity of AtOPT6, inward currents caused by KLLG and KLLLG application are 3 to 7fold greater than with KLG, overview
713255
Arabidopsis thaliana
?
-
-
-
?
7.4.2.6
additional information
ScOPT1 substrate specificity, overview. KLLLG, RPPGF, DRVYIHPF or AtCLE19p12 cause no inward currents in ScOPT1-expressing oocytes
713255
Saccharomyces cerevisiae
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4.2.6
5
-
assay at
Arabidopsis thaliana
General Information
EC Number
General Information
Commentary
Organism
7.4.2.6
physiological function
ScOPT1 is a high affinity GSH transporter. Fungal OPTs function in nutrient uptake from the environment
Saccharomyces cerevisiae
7.4.2.6
physiological function
unlike in fungi, plant OPTs do not function in nutrient uptake from the environment, but OPT6 can transport plant signaling peptides
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.4.2.6
physiological function
ScOPT1 is a high affinity GSH transporter. Fungal OPTs function in nutrient uptake from the environment
Saccharomyces cerevisiae
7.4.2.6
physiological function
unlike in fungi, plant OPTs do not function in nutrient uptake from the environment, but OPT6 can transport plant signaling peptides
Arabidopsis thaliana