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Literature summary extracted from

  • Harada, J.; Nagashima, K.V.; Takaichi, S.; Misawa, N.; Matsuura, K.; Shimada, K.
    Phytoene desaturase, CrtI, of the purple photosynthetic bacterium, Rubrivivax gelatinosus, produces both neurosporene and lycopene (2001), Plant Cell Physiol., 42, 1112-1118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.31 expression in Escherichia coli Rubrivivax gelatinosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.31 15-cis-phytoene + 3 acceptor Rubrivivax gelatinosus
-
all-trans-neurosporene + 3 reduced acceptor
-
?
1.3.99.31 15-cis-phytoene + 4 acceptor Rubrivivax gelatinosus
-
all-trans-lycopene + 4 reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.31 Rubrivivax gelatinosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.31 15-cis-phytoene + 3 acceptor
-
Rubrivivax gelatinosus all-trans-neurosporene + 3 reduced acceptor
-
?
1.3.99.31 15-cis-phytoene + 4 acceptor
-
Rubrivivax gelatinosus all-trans-lycopene + 4 reduced acceptor
-
?

General Information

EC Number General Information Comment Organism
1.3.99.31 malfunction a mutant of Rubrivivax gelatinosus lacking the crtI gene produces only phytoene, indicating that this organism has no other phytoene desaturases. When the crtI deletion mutant is complemented by the three-step phytoene desaturase of Rhodobacter capsulatus, spirilloxanthin and its precursors are not synthesized, although spheroidene and OH-spheroidene are accumulated Rubrivivax gelatinosus
1.3.99.31 physiological function the enzyme simultaneously catalyzes a three- and four-step desaturation of phytoene producing both neurosporene and lycopene Rubrivivax gelatinosus