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Literature summary extracted from

  • Rioz-Martinez, A.; Kopacz, M.; de Gonzalo, G.; Torres Pazmino, D.E.; Gotor, V.; Fraaije, M.W.
    Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase (2011), Org. Biomol. Chem., 9, 1337-1341.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.8 overexpression of PTDH-mFMO fusion protein in Escherichia coli Methylophaga sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.8 additional information preparation of self-sufficient monooxygenases by covalent coupling of mFMO with the soluble NADPHregenerating phosphite dehydrogenase, PTDH, from Pseudomonas stutzeri using a codon-optimized gene encoding a His-tagged and thermostable PTDH mutant as fusion partner. The bifunctional biocatalyst is able to use phosphite as a cheap and sacrificial substrate for recycling NADPH Methylophaga sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.8 soluble
-
Methylophaga sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.8 54000
-
2 * 54000, SDS-PAGE Methylophaga sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Methylophaga sp.
-
-
-
1.14.13.8 Methylophaga sp. SK1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.8 one-step purification of the recombinant PTDH-mFMO from Escherichia coli by nickel affinity chromatography Methylophaga sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 1-[4-(methylsulfanyl)phenyl]ethanone + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 21% R-enantiomer as product Methylophaga sp. (R,S)-1-[4-(methylsulfanyl)phenyl]ethanone S-oxide + NADP+ + H2O
-
?
1.14.13.8 1-[4-(methylsulfanyl)phenyl]ethanone + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 21% R-enantiomer as product Methylophaga sp. SK1 (R,S)-1-[4-(methylsulfanyl)phenyl]ethanone S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-(methylsulfanyl)pyridine + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. 2-(methylsulfanyl)pyridine S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-(methylsulfanyl)pyridine + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. SK1 2-(methylsulfanyl)pyridine S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-(methylsulfanyl)thiophene + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. 2-(methylsulfanyl)thiophene S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-(methylsulfanyl)thiophene + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. SK1 2-(methylsulfanyl)thiophene S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-chlorophenyl methyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 75% R-enantiomer as product Methylophaga sp. (R,S)-2-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-chlorophenyl methyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 75% R-enantiomer as product Methylophaga sp. SK1 (R,S)-2-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 2-[(methylsulfanyl)methyl]furan + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. 2-[(methylsulfanyl)methyl]furan S-oxide + NADP+ + H2O
-
?
1.14.13.8 3-chlorophenyl methyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 15% R-enantiomer as product Methylophaga sp. (R,S)-3-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 4-(methylsulfanyl)benzonitrile + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 22% S-enantiomer as product Methylophaga sp. (R,S)-4-(methylsulfanyl)benzonitrile S-oxide + NADP+ + H2O
-
?
1.14.13.8 4-(methylsulfanyl)phenol + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 81% S-enantiomer as product Methylophaga sp. (R,S)-4-(methylsulfanyl)phenol S-oxide + NADP+ + H2O
-
?
1.14.13.8 4-chlorophenyl methyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 95% S-enantiomer as product Methylophaga sp. (R,S)-4-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 benzyl ethyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. benzyl ethyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 benzyl methyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. benzyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 butyl ethyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. butyl ethyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 butyl methyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. butyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 cyclohexyl methyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. cyclohexyl methyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 ethyl phenyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. ethyl phenyl sulfoxide + NADP+ + H2O
-
?
1.14.13.8 methyl 2-phenylethyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. methyl 2-phenylethyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 methyl 4-(methylsulfanyl)phenyl ether + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 70% S-enantiomer as product Methylophaga sp. (R,S)-methyl 4-(methylsulfanyl)phenyl ether S-oxide + NADP+ + H2O
-
?
1.14.13.8 methyl 4-methylphenyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 92% S-enantiomer as product Methylophaga sp. (R,S)-methyl 4-methylphenyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 methyl 4-nitrophenyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 37% S-enantiomer as product Methylophaga sp. (R,S)-methyl 4-nitrophenyl sulfide S-oxide + NADP+ + H2O
-
?
1.14.13.8 methyl phenyl sulfide + NADPH + H+ + O2 sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 35% S-enantiomer as product Methylophaga sp. (R,S)-methyl phenyl sulfoxide + NADP+ + H2O
-
?
1.14.13.8 additional information substrate specificity of the recombinant PTDH-mFMO fusion enzyme, overview Methylophaga sp. ?
-
?
1.14.13.8 additional information substrate specificity of the recombinant PTDH-mFMO fusion enzyme, overview Methylophaga sp. SK1 ?
-
?
1.14.13.8 phenyl propyl sulfide + NADPH + H+ + O2 sulfoxidation by recombinant PTDH-mFMO fusion protein Methylophaga sp. phenyl propyl sulfide S-oxide + NADP+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.8 homodimer 2 * 54000, SDS-PAGE Methylophaga sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.13.8 FMO
-
Methylophaga sp.
1.14.13.8 mFMO
-
Methylophaga sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.8 70
-
recombinant PTDH-mFMO Methylophaga sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.8 35
-
purified recombinant PTDH-mFMO, half-life is 5 h Methylophaga sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.8 9
-
recombinant PTDH-mFMO Methylophaga sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.8 NADPH
-
Methylophaga sp.