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Literature summary extracted from

  • Pecsi, I.; Leveles, I.; Harmat, V.; Vertessy, B.G.; Toth, J.
    Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase (2010), Nucleic Acids Res., 38, 7179-7186.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 mutant enzymes H145W and H145A in complex with alpha,beta-imido-dUTP, hanging drop vapor diffusion method, using 50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 1.20-1.75 M ammonium sulfate and 10% (v/v) glycerol in a 1:1 ratio Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 F158A the mutant shows severely decreased catalytic activity compared to the wild type enzyme Homo sapiens
3.6.1.23 F158W the mutant shows decreased catalytic activity compared to the wild type enzyme Homo sapiens
3.6.1.23 H145A the mutant shows decreased catalytic activity compared to the wild type enzyme Mycobacterium tuberculosis
3.6.1.23 H145W the mutant shows decreased catalytic activity compared to the wild type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 alpha,beta-imido-dUTP
-
 Homo sapiens
3.6.1.23 alpha,beta-imido-dUTP
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.001
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
3.6.1.23 0.0036
-
 dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
3.6.1.23 0.0052
-
 dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Homo sapiens
-
-
-
3.6.1.23 Mycobacterium tuberculosis P9WNS5
-
-
3.6.1.23 Mycobacterium tuberculosis H37Rv P9WNS5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Homo sapiens dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Mycobacterium tuberculosis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 DUT
-
 Homo sapiens
3.6.1.23 DUT
-
 Mycobacterium tuberculosis
3.6.1.23 dUTPase
-
 Homo sapiens
3.6.1.23 dUTPase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 0.32
-
 dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
3.6.1.23 5.8
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
3.6.1.23 6.8
-
 dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.23 62
-
 dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
3.6.1.23 1900
-
 dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
3.6.1.23 5800
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens