Literature summary extracted from

Kota, V.; Rai, P.; Weitzel, J.M.; Middendorff, R.; Bhande, S.S.; Shivaji, S.
Role of glycerol-3-phosphate dehydrogenase 2 in mouse sperm capacitation (2010), Mol. Reprod. Dev., 77, 773-783.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.5.3	mitochondrion	the enzyme is localized to the outer membrane of the mitochondria facing the cytoplasm	Mus musculus	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.5.3	80000	-	SDS-PAGE	Mus musculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.8	Mus musculus	-	-	-
1.1.5.3	Mus musculus	Q64521	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
1.1.5.3	phosphoprotein	GPD2 is tyrosine phosphorylated only in capacitated spermatozoa of wild type mice	Mus musculus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.8	sperm	in the absence of Gpd2, hyperactivation and acrosome reaction are significantly altered, and a few changes in protein tyrosine phosphorylation are also observed during capacitation. GPD2 activity is required for generation of reactive oxygen species in mouse spermatozoa during capacitation, failing which, capacitation is impaired	Mus musculus	-
1.1.5.3	spermatozoon	-	Mus musculus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.5.3	sn-glycerol 3-phosphate + menadione	-	Mus musculus	glycerone phosphate + reduced menadione	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.5.3	glycerol-3-phosphate dehydrogenase 2	-	Mus musculus
1.1.5.3	GPD2	-	Mus musculus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.5.3	FAD	-	Mus musculus

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.1.5.3	Mus musculus	isoelectric focusing	7.1	6.7

General Information

EC Number	General Information	Comment	Organism
1.1.1.8	physiological function	in spermatozoa from homozygous Gpd2 deletion mice, in the absence of Gpd2, hyperactivation and acrosome reaction are significantly altered, and a few changes in protein tyrosine phosphorylation are also observed during capacitation. GPD2 activity is required for generation of reactive oxygen species in mouse spermatozoa during capacitation, failing which, capacitation is impaired	Mus musculus
1.1.5.3	malfunction	in the absence of Gpd2, hyperactivation and acrosome reaction are significantly altered, and a few changes in protein tyrosine phosphorylation are observed during sperm capacitation. The testicular size of Gpd2-deficient animals is about 10% smaller than in wild type animals. Gpd2-null animals show a 4% reduction in tubular diameter and about 10% reduction in numbers of both spermatocytes and spermatids per seminiferous tubule	Mus musculus
1.1.5.3	physiological function	GPD2 enables tyrosine phosphorylation during sperm capacitation. GPD2 activity is required for reactive oxygen species generation in mouse spermatozoa during capacitation	Mus musculus

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Release 2023.1 (January 2023)