BRENDA - Enzyme Database

Mild reductions in mitochondrial NAD-dependent isocitrate dehydrogenase activity result in altered nitrate assimilation and pigmentation but do not impact growth

Sienkiewicz-Porzucek, A.; Sulpice, R.; Osorio, S.; Krahnert, I.; Leisse, A.; Urbanczyk-Wochniak, E.; Hodges, M.; Fernie, A.R.; Nunes-Nesi, A.; Mol. Plant 3, 156-173 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.41
gene SlIDH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in antisense orientation in tomato plants
Solanum lycopersicum
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
additional information
transgenic tomato plants expressing the IDH gene in antisense orientation display a mild reduction in the activity of the target enzyme in the leaves but essentially no visible alteration in growth from the wild-type. Fruit size and yield are, however, reduced
Solanum lycopersicum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Solanum lycopersicum
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Solanum lycopersicum
-
2-oxoglutarate + NADH + H+ + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Solanum lycopersicum
-
gene SlIDH1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
leaf
-
Solanum lycopersicum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
713076
Solanum lycopersicum
2-oxoglutarate + NADH + H+ + CO2
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Solanum lycopersicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
gene SlIDH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in antisense orientation in tomato plants
Solanum lycopersicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Solanum lycopersicum
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
additional information
transgenic tomato plants expressing the IDH gene in antisense orientation display a mild reduction in the activity of the target enzyme in the leaves but essentially no visible alteration in growth from the wild-type. Fruit size and yield are, however, reduced
Solanum lycopersicum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Solanum lycopersicum
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Solanum lycopersicum
-
2-oxoglutarate + NADH + H+ + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
leaf
-
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
713076
Solanum lycopersicum
2-oxoglutarate + NADH + H+ + CO2
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.1.1.41
additional information
reduction of mitochondrial IDH activity has little effect on the relative electron transport or assimilation rates and a minor reduction in the maximum efficiency of PSII
Solanum lycopersicum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.41
additional information
reduction of mitochondrial IDH activity has little effect on the relative electron transport or assimilation rates and a minor reduction in the maximum efficiency of PSII
Solanum lycopersicum