BRENDA - Enzyme Database

Discovery of an operon that participates in agmatine metabolism and regulates biofilm formation in Pseudomonas aeruginosa

Williams, B.J.; Du, R.H.; Calcutt, M.W.; Abdolrasulnia, R.; Christman, B.W.; Blackwell, T.S.; Mol. Microbiol. 76, 104-119 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.12
expression in Escherichia coli
Pseudomonas aeruginosa PA14
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.12
C364G
complete loss of activity
Pseudomonas aeruginosa PA14
3.5.3.12
R3G/R4S
mutation of the twin-arginine translocation tat-sequence at the N-terminus. Mutant is hardly secreted to periplasm
Pseudomonas aeruginosa PA14
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.12
periplasm
isoform Agu2A' contains a twin-arginine translocation signal at its N-terminus and is secreted to the periplasm
Pseudomonas aeruginosa PA14
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
39900
-
x * 39900, calculated for mature protein
Pseudomonas aeruginosa PA14
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Pseudomonas aeruginosa PA14
Q02J61
isoform agu2A
-
3.5.3.12
Pseudomonas aeruginosa PA14
Q02J64
isoform agu2A'
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.5.3.12
proteolytic modification
cleavage of translocation signal
Pseudomonas aeruginosa PA14
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.12
0.01055
-
pH 9.0, 25°C
Pseudomonas aeruginosa PA14
3.5.3.12
0.015
-
pH 9.0, 37°C
Pseudomonas aeruginosa PA14
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
713057
Pseudomonas aeruginosa PA14
N-carbamoylputrescine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.12
?
x * 39900, calculated for mature protein
Pseudomonas aeruginosa PA14
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
expression in Escherichia coli
Pseudomonas aeruginosa PA14
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.12
C364G
complete loss of activity
Pseudomonas aeruginosa PA14
3.5.3.12
R3G/R4S
mutation of the twin-arginine translocation tat-sequence at the N-terminus. Mutant is hardly secreted to periplasm
Pseudomonas aeruginosa PA14
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.12
periplasm
isoform Agu2A' contains a twin-arginine translocation signal at its N-terminus and is secreted to the periplasm
Pseudomonas aeruginosa PA14
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
39900
-
x * 39900, calculated for mature protein
Pseudomonas aeruginosa PA14
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.5.3.12
proteolytic modification
cleavage of translocation signal
Pseudomonas aeruginosa PA14
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.12
0.01055
-
pH 9.0, 25°C
Pseudomonas aeruginosa PA14
3.5.3.12
0.015
-
pH 9.0, 37°C
Pseudomonas aeruginosa PA14
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
713057
Pseudomonas aeruginosa PA14
N-carbamoylputrescine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.12
?
x * 39900, calculated for mature protein
Pseudomonas aeruginosa PA14
Expression
EC Number
Organism
Commentary
Expression
3.5.3.12
Pseudomonas aeruginosa PA14
presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA' that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase
additional information
3.5.3.12
Pseudomonas aeruginosa PA14
presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase
up
General Information
EC Number
General Information
Commentary
Organism
3.5.3.12
physiological function
deletion of the agmatine operon agu2ABCA' reduces biofilm production of strain PA14 following addition of exogenous agmatine; deletion of the agmatine operon agu2ABCA', specifically its secreted product Agu2A', reduces biofilm production of strain PA14 following addition of exogenous agmatine
Pseudomonas aeruginosa PA14
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.3.12
physiological function
deletion of the agmatine operon agu2ABCA' reduces biofilm production of strain PA14 following addition of exogenous agmatine
Pseudomonas aeruginosa PA14
3.5.3.12
physiological function
deletion of the agmatine operon agu2ABCA', specifically its secreted product Agu2A', reduces biofilm production of strain PA14 following addition of exogenous agmatine
Pseudomonas aeruginosa PA14
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.5.3.12
Pseudomonas aeruginosa PA14
presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA' that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase
additional information
3.5.3.12
Pseudomonas aeruginosa PA14
presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase
up
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.136
-
agmatine
pH 9.0, 25°C
Pseudomonas aeruginosa PA14
3.5.3.12
2.624
-
agmatine
pH 9.0, 37°C
Pseudomonas aeruginosa PA14
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.136
-
agmatine
pH 9.0, 25°C
Pseudomonas aeruginosa PA14
3.5.3.12
2.624
-
agmatine
pH 9.0, 37°C
Pseudomonas aeruginosa PA14