Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Williams, B.J.; Du, R.H.; Calcutt, M.W.; Abdolrasulnia, R.; Christman, B.W.; Blackwell, T.S.
    Discovery of an operon that participates in agmatine metabolism and regulates biofilm formation in Pseudomonas aeruginosa (2010), Mol. Microbiol., 76, 104-119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.12 expression in Escherichia coli Pseudomonas aeruginosa PA14

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.12 C364G complete loss of activity Pseudomonas aeruginosa PA14
3.5.3.12 R3G/R4S mutation of the twin-arginine translocation tat-sequence at the N-terminus. Mutant is hardly secreted to periplasm Pseudomonas aeruginosa PA14

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.12 periplasm isoform Agu2A' contains a twin-arginine translocation signal at its N-terminus and is secreted to the periplasm Pseudomonas aeruginosa PA14
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.12 39900
-
 x * 39900, calculated for mature protein Pseudomonas aeruginosa PA14

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.12 Pseudomonas aeruginosa PA14 A0A0H2Z743 isoform agu2A'
-
3.5.3.12 Pseudomonas aeruginosa PA14 A0A0H2Z8C6 isoform agu2A
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.3.12 proteolytic modification cleavage of translocation signal Pseudomonas aeruginosa PA14

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.12 0.01055
-
 pH 9.0, 25°C Pseudomonas aeruginosa PA14
3.5.3.12 0.015
-
 pH 9.0, 37°C Pseudomonas aeruginosa PA14

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.12 agmatine + H2O
-
 Pseudomonas aeruginosa PA14 N-carbamoylputrescine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.12 ? x * 39900, calculated for mature protein Pseudomonas aeruginosa PA14

Synonyms

EC Number Synonyms Comment Organism
3.5.3.12 agu2A
-
 Pseudomonas aeruginosa PA14

Expression

EC Number Organism Comment Expression
3.5.3.12 Pseudomonas aeruginosa PA14 presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA' that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase additional information
3.5.3.12 Pseudomonas aeruginosa PA14 presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase up

General Information

EC Number General Information Comment Organism
3.5.3.12 physiological function deletion of the agmatine operon agu2ABCA' reduces biofilm production of strain PA14 following addition of exogenous agmatine Pseudomonas aeruginosa PA14
3.5.3.12 physiological function deletion of the agmatine operon agu2ABCA', specifically its secreted product Agu2A', reduces biofilm production of strain PA14 following addition of exogenous agmatine Pseudomonas aeruginosa PA14

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.12 0.136
-
 agmatine pH 9.0, 25°C Pseudomonas aeruginosa PA14
3.5.3.12 2.624
-
 agmatine pH 9.0, 37°C Pseudomonas aeruginosa PA14