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Literature summary extracted from
- Molybdenum and tungsten in Campylobacter jejuni: their physiological role and identification of separate transporters regulated by a single ModE-like protein (2009), Mol. Microbiol., 74, 758-771.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.3.2.5 | modA, genetic regulation of tupA/tupB and modA, overview | Campylobacter jejuni |
| 7.3.2.6 | tupA and tupB are two genes in the tungsten transport locus which are divergently transcribed, genetic regulation of tupA/tupB and modA, overview | Campylobacter jejuni |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.3.2.5 | additional information | construction of modA and modA/tupB defective mutants. When both transport systems are disrupted, formate dehydrogenase activity falls to 5% of the wild-type level, but activity is restored to near wild-type levels only with addition of 1 mM Na2WO4, supplementation with 1 mM Na2MoO4 only restores 20% of formate dehydrogenase activity | Campylobacter jejuni |
| 7.3.2.6 | additional information | construction of tupB and modA/tupB defective mutants. Supplementation with Na2MoO4 decreases formate dehydrogenase activity in tmutant upB:KAN to 37% of wild-type level, suggesting a preference of tungsten over molybdenum by Tup. When both transport systems are disrupted, formate dehydrogenase activity falls to 5% of the wild-type level, but activity is restored to near wild-type levels only with addition of 1 mM Na2WO4, supplementation with 1 mM Na2MoO4 only restores 20% of formate dehydrogenase activity | Campylobacter jejuni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.3.2.5 | ModE | represses molybdenum transport in presence of both molybdenum and tungsten | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.3.2.5 | ATP + H2O + molybdate/out | Campylobacter jejuni | - |
ADP + phosphate + molybdate/in | - |
? |  |
| 7.3.2.6 | ATP + H2O + tungstate/out | Campylobacter jejuni | - |
ADP + phosphate + tungstate/in | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.3.2.5 | Campylobacter jejuni | - |
gene modA | - |
| 7.3.2.6 | Campylobacter jejuni | - |
gene tupA | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.3.2.5 | ATP + H2O + molybdate/out | - |
Campylobacter jejuni | ADP + phosphate + molybdate/in | - |
? | |
| 7.3.2.5 | additional information | mod is primarily a molybdenum transporter that can also transport tungsten, while tup is a tungsten-specific transporter | Campylobacter jejuni | ? | - |
? | |
| 7.3.2.6 | ATP + H2O + tungstate/out | - |
Campylobacter jejuni | ADP + phosphate + tungstate/in | - |
? | |
| 7.3.2.6 | additional information | mod , EC 3.6.3.29, is primarily a molybdenum transporter that can also transport tungsten, while tup is a tungsten-specific transporter | Campylobacter jejuni | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.3.2.5 | More | molybdenum is typically transported into the cell via an ABC type transport system encoded by the modABC operon. The modA gene codes for the periplasmic binding protein, modB codes for the transmembrane protein, and modC codes for an ATP-binding protein. Campylobacter jejuni encodes an uncharacterized molybdenum transport system including modABC in addition to a unique gene Cj0302c with unknown function | Campylobacter jejuni |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.3.2.5 | molybdenum transporter | - |
Campylobacter jejuni |
| 7.3.2.6 | tungstate transporter | - |
Campylobacter jejuni |
| 7.3.2.6 | tungsten-specific transporter | - |
Campylobacter jejuni |
| 7.3.2.6 | TupA | - |
Campylobacter jejuni |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 7.3.2.5 | Campylobacter jejuni | genetic regulation of tupA and modA, overview | additional information |
| 7.3.2.6 | Campylobacter jejuni | genetic regulation of tupA and modA, overview | additional information |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.3.2.5 | metabolism | ModE-like protein regulates both transporters, repressing mod in the presence of both molybdenum and tungsten and tup only in the presence of tungsten. Like other ModE proteins, the Campylobacter jejuni ModE binds DNA through a helix-turn-helix DNA binding domain, but unlike other members of the ModE family it does not have a metal binding domain | Campylobacter jejuni |
| 7.3.2.5 | physiological function | molybdenum is typically transported into the cell via an ABC type transport system encoded by the modABC operon. The modA gene codes for the periplasmic binding protein, modB codes for the transmembrane protein, and modC codes for an ATP-binding protein. The enzyme provides molybdenum or tungsten for incorporation into the format dehydrogenase, formate dehydrogenase is active in the presence of either tungsten or molybdenum | Campylobacter jejuni |
| 7.3.2.6 | metabolism | ModE-like protein regulates both transporters, repressing mod, EC 3.6.3.29, in the presence of both molybdenum and tungsten and tup only in the presence of tungsten. Like other ModE proteins, the Campylobacter jejuni ModE binds DNA through a helix-turn-helix DNA binding domain, but unlike other members of the ModE family it does not have a metal binding domain | Campylobacter jejuni |
| 7.3.2.6 | physiological function | the enzyme provides tungsten for incorporation into the format dehydrogenase, formate dehydrogenase is active in the presence of either tungsten or molybdenum | Campylobacter jejuni |
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