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Literature summary extracted from

  • Johansen, S.K.; Maus, C.E.; Plikaytis, B.B.; Douthwaite, S.
    Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs (2006), Mol. Cell, 23, 173-182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.226 expression of recombinant tlyA in Escherichia coli markedly increases susceptibility to the drugs capreomycin and viomycin Mycobacterium tuberculosis
2.1.1.227 expression of recombinant tlyA in Escherichia coli markedly increases susceptibility to the drugs capreomycin and viomycin Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA Mycobacterium tuberculosis
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA
-
 ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA Mycobacterium tuberculosis
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.226 Mycobacterium tuberculosis
-
-
-
2.1.1.227 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA
-
 Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA
-
 ?
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA the bifunctional enzyme modifies nucleotide C1409 in helix 44 of 16S rRNA and nucleotide C1920 in helix 69 of 23S rRNA Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA the exact position of the methyl group in the wild-type rRNAs is verified by MALDI quadrupole-TOF tandem MS analysis ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA
-
 Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
 ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA the bifunctional enzyme modifies nucleotide C1409 in helix 44 of 16S rRNA and nucleotide C1920 in helix 69 of 23S rRNA Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA the exact position of the methyl group in the wild-type rRNAs is verified by MALDI quadrupole-TOF tandem MS analysis ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.226 TlyA
-
 Mycobacterium tuberculosis
2.1.1.227 TlyA
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.1.1.226 malfunction the bifunctional enzyme modifies nucleotide C1409 in helix 44 of 16S rRNA and nucleotide C1920 in helix 69 of 23S rRNA. Loss of these rRNA methylations confers resistance to capreomycin and viomycin Mycobacterium tuberculosis
2.1.1.227 malfunction the bifunctional enzyme modifies nucleotide C1409 in helix 44 of 16S rRNA and nucleotide C1920 in helix 69 of 23S rRNA. Loss of these rRNA methylations confers resistance to capreomycin and viomycin Mycobacterium tuberculosis