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Literature summary extracted from

  • Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.
    Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2 (2009), Microbiology, 155, 2078-2085.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.192 EDTA 1 mM, 105% of initial activity Geobacillus thermodenitrificans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.192 expression in Escherichia coli Geobacillus thermodenitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.192 Al3+ 1 mM, 14.7% residual activity; 1 mM, 18.9% residual activity Geobacillus thermodenitrificans
1.1.1.192 Ca2+ 1 mM, no residual activity Geobacillus thermodenitrificans
1.1.1.192 Co2+ 1 mM, 51.9% residual activity; 1 mM, 68.7% residual activity Geobacillus thermodenitrificans
1.1.1.192 Cu2+ 1 mM, 14.0% residual activity; 1 mM, 44.2% residual activity Geobacillus thermodenitrificans
1.1.1.192 K+ 1 mM, 70.3% residual activity; 1 mM, 82.3% residual activity Geobacillus thermodenitrificans
1.1.1.192 Mg2+ 1 mM, 0.3% residual activity; 1 mM, 74.3% residual activity Geobacillus thermodenitrificans
1.1.1.192 Mn2+ 1 mM, 14.3% residual activity; 1 mM, 23.4% residual activity Geobacillus thermodenitrificans
1.1.1.192 Ni2+ 1 mM, 18.2% residual activity; 1 mM, 72.7% residual activity Geobacillus thermodenitrificans
1.1.1.192 sodium dodecylsulfate 1 mM, 3% residual activity; 1 mM, no residual activity Geobacillus thermodenitrificans
1.1.1.192 Zn2+ 1 mM, 14.1% residual activity; 1 mM, 30.5% residual activity Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.192 0.84
-
 NADPH cosubstrate octanal, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 1.15
-
 octanal cosubstrate NADPH, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 1.44
-
 NAD+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 2.4
-
 NADP+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 3.88
-
 1-Octanol cosubstrate NADP+, pH 8.0, 60°C Geobacillus thermodenitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.192 Fe2+ 1 mM, 136% of initial activity Geobacillus thermodenitrificans
1.1.1.192 Fe2+ 1 mM, 168% of initial activity Geobacillus thermodenitrificans
1.1.1.192 additional information enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases Geobacillus thermodenitrificans
1.1.1.192 Na+ 1 mM, 110% of initial activity Geobacillus thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.192 41664
-
 8 * 45000, SDS-PAGE, 8 * 41664, calculated Geobacillus thermodenitrificans
1.1.1.192 45000
-
 8 * 45000, SDS-PAGE, 8 * 41664, calculated Geobacillus thermodenitrificans
1.1.1.192 90000
-
 PAGE Geobacillus thermodenitrificans
1.1.1.192 380000
-
 PAGE Geobacillus thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.192 Geobacillus thermodenitrificans A4IP64
-
-
1.1.1.192 Geobacillus thermodenitrificans A4ISB9
-
-
1.1.1.192 Geobacillus thermodenitrificans NG80-2 A4IP64
-
-
1.1.1.192 Geobacillus thermodenitrificans NG80-2 A4ISB9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.192 1,3-propanediol + 2 NAD+ + H2O about 45% of the activity with ethanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
 r
1.1.1.192 1,3-propanediol + 2 NAD+ + H2O about 5% of the activity with 1-octanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
 r
1.1.1.192 1,3-propanediol + 2 NAD+ + H2O about 45% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 ? + 2 NADH + 2 H+
-
 r
1.1.1.192 1,3-propanediol + 2 NAD+ + H2O about 5% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 ? + 2 NADH + 2 H+
-
 r
1.1.1.192 1-decanol + 2 NAD+ + H2O about 20% of the activity with ethanol Geobacillus thermodenitrificans decanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-decanol + 2 NAD+ + H2O about 50% of the activity with 1-octanol Geobacillus thermodenitrificans decanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-decanol + 2 NAD+ + H2O about 20% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 decanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-decanol + 2 NAD+ + H2O about 50% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 decanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-dodecanol + 2 NAD+ + H2O about 17% of the activity with ethanol Geobacillus thermodenitrificans dodecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-dodecanol + 2 NAD+ + H2O about 30% of the activity with 1-octanol Geobacillus thermodenitrificans dodecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-dodecanol + 2 NAD+ + H2O about 17% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 dodecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-dodecanol + 2 NAD+ + H2O about 30% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 dodecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-eicosanol + 2 NAD+ + H2O about 25% of the activity with 1-octanol Geobacillus thermodenitrificans eicosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-eicosanol + 2 NAD+ + H2O about 3% of the activity with ethanol Geobacillus thermodenitrificans eicosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-eicosanol + 2 NAD+ + H2O about 3% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 eicosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-eicosanol + 2 NAD+ + H2O about 25% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 eicosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-hexadecanol + 2 NAD+ + H2O about 10% of the activity with ethanol Geobacillus thermodenitrificans hexadecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-hexadecanol + 2 NAD+ + H2O about 25% of the activity with 1-octanol Geobacillus thermodenitrificans hexadecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-octacosanol + 2 NAD+ + H2O about 40% of the activity with ethanol Geobacillus thermodenitrificans octacosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-octacosanol + 2 NAD+ + H2O about 5% of the activity with 1-octanol Geobacillus thermodenitrificans octacosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-octadecanol + 2 NAD+ + H2O about 15% of the activity with 1-octanol Geobacillus thermodenitrificans octadecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-octadecanol + 2 NAD+ + H2O about 5% of the activity with ethanol Geobacillus thermodenitrificans octadecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-octanol + 2 NAD+ + H2O
-
 Geobacillus thermodenitrificans octanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-octanol + 2 NADP+ + H2O
-
 Geobacillus thermodenitrificans octanoic acid + 2 NADPH + 2 H+
-
 r
1.1.1.192 1-tetracosanol + 2 NAD+ + H2O about 10% of the activity with 1-octanol Geobacillus thermodenitrificans tetracosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-tetracosanol + 2 NAD+ + H2O about 30% of the activity with ethanol Geobacillus thermodenitrificans tetracosanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-tetradecanol + 2 NAD+ + H2O about 15% of the activity with ethanol Geobacillus thermodenitrificans tetradecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 1-tetradecanol + 2 NAD+ + H2O about 30% of the activity with 1-octanol Geobacillus thermodenitrificans tetradecanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 decanal + NAD+ + H+ about 10% of the activity with 1-octanol Geobacillus thermodenitrificans decanoic acid + NADH
-
 r
1.1.1.192 dodecanal + NAD+ + H+ about 10% of the activity with 1-octanol Geobacillus thermodenitrificans dodecanoic acid + NADH
-
 r
1.1.1.192 glycerol + 2 NAD+ + H2O about 45% of the activity with ethanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
 r
1.1.1.192 isopropanol + 2 NAD+ + H2O about 15% of the activity with 1-octanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
 r
1.1.1.192 additional information substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol Geobacillus thermodenitrificans ?
-
 ?
1.1.1.192 additional information substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol Geobacillus thermodenitrificans NG80-2 ?
-
 ?
1.1.1.192 octanal + NAD+ + H2O
-
 Geobacillus thermodenitrificans octanoic acid + NADH + H+
-
 r
1.1.1.192 octanal + NAD+ + H2O
-
 Geobacillus thermodenitrificans NG80-2 octanoic acid + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.192 octamer 8 * 45000, SDS-PAGE, 8 * 41664, calculated Geobacillus thermodenitrificans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.192 ADH1
-
 Geobacillus thermodenitrificans
1.1.1.192 ADH2
-
 Geobacillus thermodenitrificans
1.1.1.192 long-chain alkyl alcohol dehydrogenase
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.192 60
-
-
 Geobacillus thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.192 60
-
 14 h, more than 50% of initial activity Geobacillus thermodenitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.192 38.5
-
 NAD+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 283.1
-
 NADP+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 4808
-
 NADPH cosubstrate octanal, pH 8.0, 60°C Geobacillus thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.192 8
-
-
 Geobacillus thermodenitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.192 6 9
-
 Geobacillus thermodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.192 NAD+ NAD+ is prefered over NADP+ Geobacillus thermodenitrificans
1.1.1.192 NAD+ NADP+ is prefered over NAD+ Geobacillus thermodenitrificans
1.1.1.192 NADH
-
 Geobacillus thermodenitrificans
1.1.1.192 NADP+ NAD+ is prefered over NADP+ Geobacillus thermodenitrificans
1.1.1.192 NADP+ NADP+ is prefered over NAD+ Geobacillus thermodenitrificans
1.1.1.192 NADPH
-
 Geobacillus thermodenitrificans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.192 26.7
-
 NAD+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 67.2
-
 1-Octanol cosubstrate NADP+, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 117.9
-
 NADP+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 450.8
-
 octanal cosubstrate NADPH, pH 8.0, 60°C Geobacillus thermodenitrificans
1.1.1.192 5724
-
 NADPH cosubstrate octanal, pH 8.0, 60°C Geobacillus thermodenitrificans