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Literature summary extracted from

  • Abad, S.; Nahalka, J.; Bergler, G.; Arnold, S.A.; Speight, R.; Fotheringham, I.; Nidetzky, B.; Glieder, A.
    Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst (2010), Microb. Cell Fact., 9, 24.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.4.3.3 biotechnology the enzyme is used as a biocatalyst in cephalosporin C conversion on industrial scale Trigonopsis variabilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 stable expression in Pichia pastoris with 7fold enhancement of the intracellular level of oxidase activity, expression optimization by codon redesign as well as efficient subcellular targeting of the enzyme to peroxisomes Trigonopsis variabilis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 additional information development of a robust and highly active Pichia pastoris TvDAO whole-cell biocatalyst in a multistep engineering process for use in cephalosporin C conversion on industrial scale, overview. Usage of a fed-batch cultivation of the multicopy strain Trigonopsis variabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-amino acid + H2O + O2 Trigonopsis variabilis
-
 2-oxo acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Trigonopsis variabilis Q6R4Q9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 additional information
-
 intracellular activity of recombinant DAO in transformed Pichia pastoris, overview Trigonopsis variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-amino acid + H2O + O2
-
 Trigonopsis variabilis 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.3 D-amino acid + H2O + O2 via formation of an imino acid Trigonopsis variabilis 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.3 additional information conversion of cephalosporine C to 7-aminocephalosporanic acid and spontaneous decarboxylation of oxoadipyl-7-amino cephalosporanic acid is promoted by the H2O2 formed in the oxidase reaction of TvDAO Trigonopsis variabilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 DAO
-
 Trigonopsis variabilis