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Literature summary extracted from

  • Semba, H.; Ichige, E.; Imanaka, T.; Atomi, H.; Aoyagi, H.
    Efficient production of active form recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture (2010), Methods Mol. Biol., 643, 133-144.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.47 synthesis hydroxynitrile lyase is a useful enzyme for production of optically active cyanohydrin compounds Manihot esculenta

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.47 expressed in Escherichia coli BL21(DE3) cells Manihot esculenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.47 30000
-
 recombinant enzyme, SDS-PAGE Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta P52705 cassava
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.47 leaf
-
 Manihot esculenta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 DL-mandelonitrile
-
 Manihot esculenta benzaldehyde + HCN
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 EC 4.1.2.39 formerly Manihot esculenta
4.1.2.47 HNL
-
 Manihot esculenta
4.1.2.47 Hydroxynitrile lyase
-
 Manihot esculenta

Expression

EC Number Organism Comment Expression
4.1.2.47 Manihot esculenta the enzyme activity and yield at low-temperature cultures (17°C) are 850times higher than those obtained at the optimum growth temperature of 37°C up