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Literature summary extracted from

  • Takenoya, M.; Ohtaki, A.; Noguchi, K.; Endo, K.; Sasaki, Y.; Ohsawa, K.; Yajima, S.; Yohda, M.
    Crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase from the hyperthermophile Thermotoga maritima for insights into the coordination of conformational changes and an inhibitor binding (2010), J. Struct. Biol., 170, 532-539.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.267 in complex with NADPH and Mg2+, and in complex with fosmidomycin, NADPH and Mg2+, both to 2.0 A resolution Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 fosmidomycin
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.0028
-
 NADPH pH 7.5, 50°C Thermotoga maritima
1.1.1.267 0.0105
-
 NADPH pH 7.5, 85°C Thermotoga maritima
1.1.1.267 0.04
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 50°C Thermotoga maritima
1.1.1.267 0.11
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 85°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ Mg2+ is prefered over Mn2+ Thermotoga maritima
1.1.1.267 Mn2+ Mg2+ is prefered over Mn2+ Thermotoga maritima
1.1.1.267 additional information divalent cation is required Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Thermotoga maritima Q9WZZ1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.267 3
-
 presence of Mn2+, 50°C, pH 7.5 Thermotoga maritima
1.1.1.267 5
-
 presence of Mg2+, 50°C, pH 7.5 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Thermotoga maritima 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 70
-
 optimum in presence of Mn2+ Thermotoga maritima
1.1.1.267 85
-
 or above, optimum in presence of Mg2+ Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.267 30
-
 almost complete loss of activity below Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 0.29
-
 NADPH pH 7.5, 50°C Thermotoga maritima
1.1.1.267 5.5
-
 NADPH pH 7.5, 85°C Thermotoga maritima

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.267 93
-
 pH 7.5, 50°C Thermotoga maritima fosmidomycin
1.1.1.267 107
-
 pH 7.5, 85°C Thermotoga maritima fosmidomycin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.267 7.5
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 50°C Thermotoga maritima
1.1.1.267 50
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 85°C Thermotoga maritima