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Literature summary extracted from

  • Luo, H.B.; Zheng, H.; Zimmerman, M.D.; Chruszcz, M.; Skarina, T.; Egorova, O.; Savchenko, A.; Edwards, A.M.; Minor, W.
    Crystal structure and molecular modeling study of N-carbamoylsarcosine amidase Ta0454 from Thermoplasma acidophilum (2010), J. Struct. Biol., 169, 304-311.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.59 the small regulatory subunit is cloned, expressed and purified Thermoplasma acidophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.59 structure solved and refined at a resolution of 2.35 A. The enzyme shares a similar fold and a highly conserved C-D-K-catalytic triad, Cys123, Asp9, and Lys90 with the structures of three cysteine hydrolases (PDB codes: 1NBA, 1IM5, and 2HOR). Molecular dynamics simulations of Ta0454/N-carbamoylsarcosine and Ta0454/pyrazinamide complexes are performed to determine the structural basis of the substrate binding pattern for each ligand. The predicted binding free energies suggest that Ta0454 is selective for N-carbamoylsarcosine over pyrazinamide, and zinc ions play an important role in the favorable substrate bound states Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.59 Zinc zinc ions play an important role in the favorable substrate bound staes Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.59 Thermoplasma acidophilum Q9HKY9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.59 the small regulatory subunit is cloned, expressed and purified Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.59 N-carbamoylsarcosine + H2O
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 Thermoplasma acidophilum sarcosine + CO2 + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.59 CSHase
-
 Thermoplasma acidophilum
3.5.1.59 N-carbamoylsarcosine amidase Ta0454
-
 Thermoplasma acidophilum

General Information

EC Number General Information Comment Organism
3.5.1.59 physiological function involved in the degradation of creatinine Thermoplasma acidophilum