EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.5.1 | gene encoding DHAr, DNA and amino acid sequence determination and analysis, phylogenetic tree | Malpighia glabra |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.5.1 | cytosol | - |
Malpighia glabra | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.5.1 | 2 glutathione + dehydroascorbate | Malpighia glabra | - |
glutathione disulfide + ascorbate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.5.4 | Malpighia glabra | E2RWY5 | acerola | - |
1.8.5.1 | Malpighia glabra | E2RWY6 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.5.4 | fruit | MDHAR activity increases gradually and significantly as ripening progresses,with overripe fruits having the highest activity | Malpighia glabra | - |
1.6.5.4 | leaf | - |
Malpighia glabra | - |
1.8.5.1 | fruit | ripening, expression analysis, transcript level of DHAR is highest at the intermediate stage of fruit ripening, overview | Malpighia glabra | - |
1.8.5.1 | leaf | - |
Malpighia glabra | - |
1.8.5.1 | additional information | under dark conditions, there is a sharp and significant decline in the total and reduced ascorbate contents, accompanied by a decrease in the level of transcripts and enzyme activities of the two genes in acerola leaves | Malpighia glabra | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.5.4 | NADH + monodehydroascorbate | - |
Malpighia glabra | NAD+ + ascorbate | - |
? | |
1.8.5.1 | 2 glutathione + dehydroascorbate | - |
Malpighia glabra | glutathione disulfide + ascorbate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.6.5.4 | MDHAR | - |
Malpighia glabra |
1.8.5.1 | dehydroascorbate reductase | - |
Malpighia glabra |
1.8.5.1 | DHAR | - |
Malpighia glabra |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 25 | - |
assay at | Malpighia glabra |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 7 | - |
assay at | Malpighia glabra |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.5.4 | NADH | - |
Malpighia glabra |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.6.5.4 | Malpighia glabra | MDHAR transcripts and enzyme activities are significantly up-regulated in the leaves of acerola under cold (4°C for 24 h) and salt (treatment with 150 mM NaCl over 9 days) stress conditions. MDHAR activity increases gradually and significantly as ripening progresses,with overripe fruits having the highest activity | up |
1.8.5.1 | Malpighia glabra | under dark conditions, there is a sharp and significant decline in the total and reduced ascorbate contents, accompanied by a decrease in the level of transcripts and enzyme activities of the two genes in acerola leaves | down |
1.8.5.1 | Malpighia glabra | DHAR transcript and enzyme activity are significantly up-regulated in the leaves of acerola under cold and salt stress conditions | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.5.1 | additional information | transcriptional regulation of DHAR, overview | Malpighia glabra |
1.8.5.1 | physiological function | monodehydroascorbate reductase and dehydroascorbate reductase are key enzymes of the ascorbate-glutathione cycle that maintain reduced pools of ascorbic acid and serve as important antioxidants | Malpighia glabra |