Literature summary extracted from

Huang, S.; Hsu, Y.; Wu, C.; Lynn, J.; Li, W.
Thermal effects on the activity and structural conformation of catechol 2,3-dioxygenase from Pseudomonas putida SH1 (2010), J. Phys. Chem. B, 114, 987-992.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.13.11.2	Fe2+	the enzyme contains Fe2+ as a cofactor	Pseudomonas putida

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.13.11.2	39000	-	4 * 39000, SDS-PAGE	Pseudomonas putida
1.13.11.2	128000	-	gel filtration	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.2	Pseudomonas putida	P06622	-	-
1.13.11.2	Pseudomonas putida SH1	P06622	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.13.11.2	DEAE-Sepharose column chromatography, phenyl-Superose gel filtration, and Sephacryl S-200 gel filtration	Pseudomonas putida

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.13.11.2	culture condition:naphthalene-grown cell	-	Pseudomonas putida	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.13.11.2	15	-	crude extract, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C	Pseudomonas putida
1.13.11.2	802	-	after 52.7fold purification, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C	Pseudomonas putida

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.11.2	catechol + O2	-	Pseudomonas putida	2-hydroxymuconate semialdehyde	-	?
1.13.11.2	catechol + O2	-	Pseudomonas putida SH1	2-hydroxymuconate semialdehyde	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.13.11.2	homotetramer	4 * 39000, SDS-PAGE	Pseudomonas putida

Synonyms

EC Number	Synonyms	Comment	Organism
1.13.11.2	C23o	-	Pseudomonas putida

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.13.11.2	50	-	-	Pseudomonas putida

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
1.13.11.2	25	80	the enzyme activity increases starting from 25°C and reaches its maximum at 50°C, the enzyme starts to denature at 55°C and the structure is destroyed by the time the temperature reaches 80°C	Pseudomonas putida

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.13.11.2	30	50	the half-life of the enzyme activity at 30°C is about 7 h and the half-life of the enzyme activity at 50°C is 45 min	Pseudomonas putida

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.13.11.2	7.5	8	-	Pseudomonas putida

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Release 2023.1 (January 2023)