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Literature summary extracted from
- Immobilization of catalase onto Eupergit C and its characterization (2010), J. Mol. Catal. B, 64, 177-183.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.11.1.6 | free and immobilized catalases show their maximum activities at 50 mM buffer concentration. When the buffer concentration is increased from 25 to 100 mM, the activity of Eupergit C-immobilized catalase is more affected than the activity of free catalase. At 100 mM buffer concentration, free catalase retains 96.9% of its maximum activity although immobilized catalase retains 80% of its maximum activity | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.6 | 28.6 | - |
H2O2 | free catalase, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus |  |
| 1.11.1.6 | 95.9 | - |
H2O2 | catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.6 | Bos taurus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.11.1.6 | liver | - |
Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.11.1.6 | 22°C and 5°C, free enzyme, 11 days, 32% loss of activity | Bos taurus |
| 1.11.1.6 | 22°C, Eupergit C-immobilized enzyme, 11 days, 21% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.6 | H2O2 | - |
Bos taurus | O2 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.6 | CAT | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 25 | - |
free enzyme | Bos taurus |
| 1.11.1.6 | 40 | - |
Eupergit C-immobilized catalase | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 40 | 50 | the half lives of free catalase at 40 and 50°C are 9.0 and 6.7 h, respectively, and correspondingly 29.1 and 4.8 h for Eupergit C-immobilized catalase | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 7 | - |
Eupergit C-immobilized enzyme | Bos taurus |
| 1.11.1.6 | 7.5 | - |
free enzyme | Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 6 | 7.5 | the activity of Eupergit C-immobilized catalase remains almost constant for pH values 6.0-7.5 whereas this is not case for the free catalase | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.6 | heme | - |
Bos taurus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.11.1.6 | Bos taurus | isoelectric focusing | - |
5.4 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.6 | 160 | - |
H2O2 | catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus | |
| 1.11.1.6 | 21000 | - |
H2O2 | free catalase, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus | |
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Release 2023.1 (January 2023)
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