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Literature summary extracted from

  • Pchelintsev, N.; Youshko, M.; Švedas, V.
    Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase (2009), J. Mol. Catal. B, 56, 202-207.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.11 additional information immobilization of the enzyme, quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase under different conditions by confocal fluorescent microscopy, overview. Comparison of fresh aggregates and mature aggregates. The aggregate size might regulate the extent of covalent modification of the enzyme and thereby influence the catalytic properties of cross-linked enzyme aggregates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.11 PMSF irreversible, used for active site titrations of the cross-linked enzyme aggregates Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 0.07
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, native enzyme Escherichia coli
3.5.1.11 0.14
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, fresh immobilized cross-linked enzyme aggregates Escherichia coli
3.5.1.11 0.23
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, mature immobilized cross-linked enzyme aggregates Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 5-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
 Escherichia coli phenylacetate + 3-amino-5-nitro-benzoic acid
-
 ?
3.5.1.11 penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanic acid + phenyl acetic acid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.11 0.9
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, fresh immobilized cross-linked enzyme aggregates Escherichia coli
3.5.1.11 1
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, mature immobilized cross-linked enzyme aggregates Escherichia coli
3.5.1.11 1.2
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, native enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 7 7.5 assay at Escherichia coli