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Literature summary extracted from

  • Shakeri, M.; Shoda, M.
    Decolorization of an anthraquinone dye by the recombinant dye-decolorizing peroxidase (rDyP) immobilized on mesoporous materials (2008), J. Mol. Catal. B, 54, 42-49.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Aspergillus oryzae strain RD005 Thanatephorus cucumeris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.19 58000
-
 gel filtration Thanatephorus cucumeris

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Thanatephorus cucumeris
-
 strain Dec 1, formerly Geotrichum candidum Dec 1
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.11.1.19 immobilized recombinant DyP has higher stability to H2O2 compared to free recombinant DyP. The stability of recombinant DyP immobilized on FSM-16 at pH 4.0 and on AlSBA-15 at pH 3.0 and 4 are similar to that of free recombinant DyP Thanatephorus cucumeris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 ammonium sulfate precipitation and butyl-Toyopearl column chromatography Thanatephorus cucumeris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 Reactive Blue 5 + H2O2 + H+
-
 Thanatephorus cucumeris oxidized Reactive Blue 5 + H2O
-
 ?
1.11.1.19 Remazol Brilliant Blue R + H2O2 + H+
-
 Thanatephorus cucumeris oxidized Remazol Brilliant Blue R + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Thanatephorus cucumeris
1.11.1.19 DyP
-
 Thanatephorus cucumeris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 4
-
 overall efficiency is maximum for immobilized recombinant DyP on AlSBA-15 at pH 4.0 Thanatephorus cucumeris
1.11.1.19 5
-
 overall efficiency is maximum for immobilized recombinant DyP on FSM-16 at pH 5.0 Thanatephorus cucumeris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.19 3 6 more than 90% of initial free recombinant DyP activity remains active after incubation in citrate buffered solution at pH 3.0-5.0 for 48 h. Adsorption yields of recombinant DyP immobilized on FSM-16 and AlSBA-15 increases as pH decreased from 6.0 to 3.0, however, the activity yield of immobilized recombinant DyP decreases with decreasing pH Thanatephorus cucumeris