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Literature summary extracted from

  • Kumar, P.; Mohammadi, M.; Viger, J.F.; Barriault, D.; Gomez-Gil, L.; Eltis, L.D.; Bolin, J.T.; Sylvestre, M.
    Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution (2011), J. Mol. Biol., 405, 531-547.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 expressed in Escherichia coli DH11S cells Paraburkholderia xenovorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.18 sitting drop vapor diffusion method, using 20–25% (w/v) PEG 8000 or PEG 5000 MME, 50 mM PIPES (pH 6.5), 100 mM ammonium acetate, 5% (v/v) glycerol, and 0.2% (w/v) agarose Paraburkholderia xenovorans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.18 F336M the mutant produces principally 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl from 2,2'-dichlorobiphenyl Paraburkholderia xenovorans
1.14.12.18 T335A the mutant yields 2,3-dihydroxy-2,2'chlorobiphenyl as the major metabolite from 2,2'-dichlorobiphenyl Paraburkholderia xenovorans
1.14.12.18 T335A/F336M the mutant metabolizes 2,2'-dichlorobiphenyl better than the wild type enzyme to generate principally 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl instead of 2,3-dihydroxy-2'-chlorobiphenyl as produced by the wild type enzyme, the mutant produces 3',4'-dihydroxy-3',4'-dihydro-2,6-dichlorobiphenyl as a major metabolite from 2,6-dichlorobiphenyl Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.18 biphenyl + NADH + H+ + O2 Paraburkholderia xenovorans
-
 cis-(2R,3S)-dihydroxy-1-phenylcyclohexa-4,6-diene + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.18
-
 Paraburkholderia xenovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 2,2'-dichlorobiphenyl + NADH + H+ + O2 poor substrate Paraburkholderia xenovorans 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,6-dichlorobiphenyl + NADH + H+ + O2 poor substrate Paraburkholderia xenovorans ?
-
 ?
1.14.12.18 biphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans cis-(2R,3S)-dihydroxy-1-phenylcyclohexa-4,6-diene + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 BPDO
-
 Paraburkholderia xenovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 NADH
-
 Paraburkholderia xenovorans

General Information

EC Number General Information Comment Organism
1.14.12.18 metabolism BPDO is one of the most potent biocatalysts of natural origin for the dioxygenation of chlorobiphenyls Paraburkholderia xenovorans