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Literature summary extracted from

  • Sendovski, M.; Kanteev, M.; Ben-Yosef, V.S.; Adir, N.; Fishman, A.
    First structures of an active bacterial tyrosinase reveal copper plasticity (2011), J. Mol. Biol., 405, 227-237.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.1 expressed in Escherichia coli BL21 cells Priestia megaterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.18.1 hanging drop vapor diffusion method Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.1 R209H the mutant possesses a higher monophenolase/diphenolase activity ratio than the wild type enzyme Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 kojic acid i.e. 5-hydroxy-2-(hydroxymethyl)-g-pyrone Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ tyrosinase is a member of the type 3 copper enzyme family, two Cu2+ ions serveas the major cofactors within the active site Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 35000
-
 2 * 35000, X-ray crystallography Priestia megaterium
1.14.18.1 70000
-
 gel filtration Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Priestia megaterium B2ZB02
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-tyrosine + O2
-
 Priestia megaterium dopachrome + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 homodimer 2 * 35000, X-ray crystallography Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyr
-
 Priestia megaterium
1.14.18.1 tyrosinase
-
 Priestia megaterium