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Literature summary extracted from

  • Richter, N.; Breicha, K.; Hummel, W.; Niefind, K.
    The Three-Dimensional Structure of AKR11B4, a Glycerol Dehydrogenase from Gluconobacter oxydans, Reveals a Tryptophan Residue as an Accelerator of Reaction Turnover (2010), J. Mol. Biol., 404, 353-362.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.72 His-tagged version Gluconobacter oxydans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.72 sitting-drop variant of the vapor diffusion method Gluconobacter oxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.72 W23A W23 is a key residue (accelerator) for NADP/NADPH turnover Gluconobacter oxydans
1.1.1.72 W23M W23 is a key residue (accelerator) for NADP/NADPH turnover Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.72 additional information
-
 additional information KM-value 118.0 mM with substrate DL-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 additional information
-
 additional information KM-value 2.4 mM with substrate DL-glyceraldehyde, wild-type protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 additional information
-
 additional information KM-value 49.4 mM with substrate DL-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 0.0041
-
 NADPH wild-type protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 0.0062
-
 NADPH W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 0.0062
-
 NADPH W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.72 37213
-
 37213, calculated Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.72 Gluconobacter oxydans Q5FQJ0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.72
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.72 4.2
-
 W23A mutant protein, NADPH as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 4.9
-
 W23A mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 37
-
 W23M mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 54
-
 W23M mutant protein, NADPH as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 80.5
-
 wild-type protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 87.1
-
 wild-type protein, NADPH as variable substrate, pH 7.0, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.72 DL-glyceraldehyde + NADPH + H+ significant enantioselectivity Gluconobacter oxydans glycerol + NADP+
-
 r
1.1.1.72 glycerol + NADP+
-
 Gluconobacter oxydans D-glyceraldehyde + NADPH + H+ significant enantioselectivity r

Subunits

EC Number Subunits Comment Organism
1.1.1.72 monomer 37213, calculated Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.72 AKR11B4
-
 Gluconobacter oxydans
1.1.1.72 glycerol dehydrogenase
-
 Gluconobacter oxydans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.72 additional information
-
 additional information 24.2/sec with substrate D/L-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 additional information
-
 additional information 3.2/sec with substrate D/L-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 additional information
-
 additional information 52.8/sec with substrate D/L-glyceraldehyde, wild-type protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 2.7
-
 NADPH W23A mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 35.4
-
 NADPH W23M mutant protein, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.72 57.2
-
 NADPH wild-type protein, pH 7.0, 30°C Gluconobacter oxydans