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Literature summary extracted from

  • Tylichova, M.; Kopecny, D.; Morera, S.; Briozzo, P.; Lenobel, R.; Snegaroff, J.; Sebela, M.
    Structural and functional characterization of plant aminoaldehyde dehydrogenase from Pisum sativum with a broad specificity for natural and synthetic aminoaldehydes (2010), J. Mol. Biol., 396, 870-882.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.19
-
 Pisum sativum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.19 isozyme AMADH1 in complex with NAD+, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 13% (w/v) PEG 6000 and 5% (v/v) 2-methyl-2,4-pentanediol, at 20°C Pisum sativum
1.2.1.19 isozyme AMADH2 in complex with NAD+, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 18% (w/v) PEG 4000, 10% (v/v) isopropanol and 0.5% (w/v) n-octyl beta-D-glucopyranoside, at 20°C Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.19 0.007
-
 4-guanidinobutyraldehyde isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.01
-
 N,N,N-trimethyl-4-aminobutyraldehyde isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.01
-
 3-aminopropionaldehyde isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.011
-
 4-guanidinobutyraldehyde isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.021
-
 N,N,N-trimethyl-4-aminobutyraldehyde isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.029
-
 4-Aminobutyraldehyde isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.04
-
 NAD+ isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.055
-
 NAD+ isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.075
-
 3-aminopropionaldehyde isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum
1.2.1.19 0.17
-
 4-Aminobutyraldehyde isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.19 117000
-
 gel filtration Pisum sativum
1.2.1.19 120000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Pisum sativum Q8VWZ1 isozyme AMADH1
-
1.2.1.19 Pisum sativum Q93YB2 isozyme AMADH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 cobalt- or nickel-charged IDA-Sepharose column chromatography and Resource Q column chromatography Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 3-aminopropionaldehyde + NAD+ + H2O 100% activity Pisum sativum 3-aminopropanoate + NADH + H+
-
 ?
1.2.1.19 3-cyanopropionaldehyde + NAD+ + H2O 108% activity compared to 3-aminopropionaldehyde Pisum sativum 3-cyanopropanoate + NADH + H+
-
 ?
1.2.1.19 3-cyanopropionaldehyde + NAD+ + H2O 99% activity compared to 3-aminopropionaldehyde Pisum sativum 3-cyanopropanoate + NADH + H+
-
 ?
1.2.1.19 3-guanidinopropionaldehyde + NAD+ + H2O 12% activity compared to 3-aminopropionaldehyde Pisum sativum 3-guanidinopropanoate + NADH + H+
-
 ?
1.2.1.19 3-guanidinopropionaldehyde + NAD+ + H2O 17% activity compared to 3-aminopropionaldehyde Pisum sativum 3-guanidinopropanoate + NADH + H+
-
 ?
1.2.1.19 4-amino-2-hydroxybutyraldehyde + NAD+ + H2O 13% activity compared to 3-aminopropionaldehyde Pisum sativum 4-amino-2-hydroxybutanoate + NADH + H+
-
 ?
1.2.1.19 4-amino-2-hydroxybutyraldehyde + NAD+ + H2O 20% activity compared to 3-aminopropionaldehyde Pisum sativum 4-amino-2-hydroxybutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutyraldehyde + NAD+ + H2O 36% activity compared to 3-aminopropionaldehyde Pisum sativum 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutyraldehyde + NAD+ + H2O 82% activity compared to 3-aminopropionaldehyde Pisum sativum 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-guanidino-2-hydroxybutyraldehyde + NAD+ + H2O 22% activity compared to 3-aminopropionaldehyde Pisum sativum 4-guanidino-2-hydroxybutanoate + NADH + H+
-
 ?
1.2.1.19 4-guanidino-2-hydroxybutyraldehyde + NAD+ + H2O 25% activity compared to 3-aminopropionaldehyde Pisum sativum 4-guanidino-2-hydroxybutanoate + NADH + H+
-
 ?
1.2.1.19 4-guanidinobutyraldehyde + NAD+ + H2O 29% activity compared to 3-aminopropionaldehyde Pisum sativum 4-guanidinobutanoate + NADH + H+
-
 ?
1.2.1.19 4-guanidinobutyraldehyde + NAD+ + H2O 30% activity compared to 3-aminopropionaldehyde Pisum sativum 4-guanidinobutanoate + NADH + H+
-
 ?
1.2.1.19 additional information 3-acetylpyridine-NAD+ is the best electron acceptor and leads to 33% activity compared to that with NAD+, while thio-NAD+ drastically and especially affects isozyme AMADH1 activity. 3-Pyridinealdehyde-NAD+ hardly functions as a coenzyme for AMADH1. Deamino-NAD+ is a better coenzyme than NAD+ and increases the reaction rate by 52% Pisum sativum ?
-
 ?
1.2.1.19 additional information 3-acetylpyridine-NAD+ is the best electron acceptor and leads to 33% activity compared to that with NAD+, while thio-NAD+ drastically and especially affects isozyme AMADH1 activity. 3-Pyridinealdehyde-NAD+ hardly functions as a coenzyme for AMADH1. Deamino-NAD+ is a better coenzyme than NAD+ and increases the reaction rate by 72% Pisum sativum ?
-
 ?
1.2.1.19 additional information isozyme AMADH1 preferentially oxidizes C3 and C4 aminoaldehydes and has no activity with butyraldehyde, acetaldehyde, propionaldehyde, betaine aldehyde, valeraldehyde, capronaldehyde, enanthaldehyde, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, and 4-pyridine carboxaldehyde Pisum sativum ?
-
 ?
1.2.1.19 additional information isozyme AMADH2 preferentially oxidizes C3 and C4 aminoaldehydes and has no activity with butyraldehyde, acetaldehyde, propionaldehyde, betaine aldehyde, valeraldehyde, capronaldehyde, enanthaldehyde, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, and 4-pyridine carboxaldehyde Pisum sativum ?
-
 ?
1.2.1.19 N,N,N-trimethyl-3-aminopropionaldehyde + NAD+ + H2O 35% activity compared to 3-aminopropionaldehyde Pisum sativum gamma-butyrobetaine + NADH + H+
-
 ?
1.2.1.19 N,N,N-trimethyl-3-aminopropionaldehyde + NAD+ + H2O 54% activity compared to 3-aminopropionaldehyde Pisum sativum gamma-butyrobetaine + NADH + H+
-
 ?
1.2.1.19 N,N,N-trimethyl-4-aminobutyraldehyde + NAD+ + H2O 45% activity compared to 3-aminopropionaldehyde Pisum sativum N,N,N-trimethyl-4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 N,N,N-trimethyl-4-aminobutyraldehyde + NAD+ + H2O 48% activity compared to 3-aminopropionaldehyde Pisum sativum N,N,N-trimethyl-4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 N,N-dimethyl-4-aminobutyraldehyde + NAD+ + H2O 57% activity compared to 3-aminopropionaldehyde Pisum sativum N,N-dimethyl-4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 N,N-dimethyl-4-aminobutyraldehyde + NAD+ + H2O 80% activity compared to 3-aminopropionaldehyde Pisum sativum N,N-dimethyl-4-aminobutanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.19 dimer x-ray crystallography Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.19 4-aminobutyraldehyde dehydrogenase
-
 Pisum sativum
1.2.1.19 ABALDH
-
 Pisum sativum
1.2.1.19 AMADH1 isozyme Pisum sativum
1.2.1.19 AMADH2 isozyme Pisum sativum
1.2.1.19 aminoaldehyde dehydrogenase
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+ preferred cofactor over NADP+ Pisum sativum
1.2.1.19 NADP+ NADP+ is a poor electron acceptor compared to NAD+ reducing the activity of isozyme AMDH1 by 86% Pisum sativum
1.2.1.19 NADP+ NADP+ is a poor electron acceptor compared to NAD+ reducing the activity of isozyme AMDH2 by 85% Pisum sativum

General Information

EC Number General Information Comment Organism
1.2.1.19 metabolism AMADH participates in carnitine biosynthesis in plants Pisum sativum