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Literature summary extracted from

  • Zou, P.; McHaourab, H.S.
    Alternating access of the putative substrate-binding chamber in the ABC transporter MsbA (2009), J. Mol. Biol., 393, 574-585.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
7.5.2.6 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
7.5.2.6 ATP + H2O + lipopolysaccharide[side 1] = ADP + phosphate + lipopolysaccharide[side 2] spin labeling and electron paramagnetic resonance spectroscopy are used to systematically explore rearrangements in MsbA structure during the ATP hydrolysis cycle. Spin-label accessibility and local dynamics are determined in liposomes for the nucleotide-free intermediate and the transition state of ATP hydrolysis. The changes in the electron paramagnetic resonance parameters between these two intermediates fit a global pattern consistent with alternating access of the chamber. In the transition state of ATP hydrolysis, spin labels on the cytoplasmic side report increase dynamic restrictions and reduce water accessibility, while those on the extracellular side report increase water penetration Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.5.2.6 ATP + H2O + Lipid A/in
-
 Escherichia coli ADP + phosphate + Lipid A/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.5.2.6 MsbA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5.2.6 37
-
 assay at Escherichia coli