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Literature summary extracted from

  • Schmitt, E.; Galimand, M.; Panvert, M.; Courvalin, P.; Mechulam, Y.
    Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases (2009), J. Mol. Biol., 388, 570-582.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.179
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.179 RmtB containing S-adenosyl-L-homocysteine in the active site Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.179 E182A tobramycin MIC is identical with that of wild-type RmtB Escherichia coli
2.1.1.179 H50A tobramycin MIC is drastically reduced compared to wild-type enzyme Escherichia coli
2.1.1.179 H81A tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme Escherichia coli
2.1.1.179 K14A tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme Escherichia coli
2.1.1.179 K174A tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme Escherichia coli
2.1.1.179 R17A tobramycin MIC is identical with that of wild-type RmtB Escherichia coli
2.1.1.179 R181A tobramycin MIC is drastically reduced compared to wild-type enzyme Escherichia coli
2.1.1.179 R48A tobramycin MIC is identical with that of wild-type RmtB Escherichia coli
2.1.1.179 S83A tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme Escherichia coli
2.1.1.179 Y56F tobramycin MIC is identical with that of wild-type RmtB Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA Escherichia coli post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.179 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.179
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits Escherichia coli S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.179 RmtB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.179 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.179 7.5
-
 assay at Escherichia coli