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Literature summary extracted from

  • Park, Y.M.; Phi, Q.T.; Song, B.H.; Ghim, S.Y.
    Thermostability of chimeric cytidine deaminase variants produced by DNA shuffling (2009), J. Microbiol. Biotechnol., 19, 1536-1541.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 expressed in Escherichia coli Rosetta(DE3)pLysS cells Geobacillus stearothermophilus
3.5.4.5 expressed in Escherichia coli Rosetta(DE3)pLysS cells [Bacillus] caldolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 14000
-
 4 * 14000, SDS-PAGE Geobacillus stearothermophilus
3.5.4.5 14000
-
 4 * 14000, SDS-PAGE [Bacillus] caldolyticus
3.5.4.5 16000
-
 His-tagged CDA, SDS-PAGE Geobacillus stearothermophilus
3.5.4.5 16000
-
 His-tagged CDA, SDS-PAGE [Bacillus] caldolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Geobacillus stearothermophilus
-
-
-
3.5.4.5 Geobacillus stearothermophilus IFO12550 (T101)
-
-
-
3.5.4.5 [Bacillus] caldolyticus
-
-
-
3.5.4.5 [Bacillus] caldolyticus DSM 405 (T53)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5 Ni-NTA column chromatography Geobacillus stearothermophilus
3.5.4.5 Ni-NTA column chromatography [Bacillus] caldolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 cytidine + H2O
-
 Geobacillus stearothermophilus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 [Bacillus] caldolyticus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Geobacillus stearothermophilus IFO12550 (T101) uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 [Bacillus] caldolyticus DSM 405 (T53) uridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 homotetramer 4 * 14000, SDS-PAGE Geobacillus stearothermophilus
3.5.4.5 homotetramer 4 * 14000, SDS-PAGE [Bacillus] caldolyticus

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 CDA
-
 Geobacillus stearothermophilus
3.5.4.5 CDA
-
 [Bacillus] caldolyticus
3.5.4.5 cytidine/2'-deoxycytidine aminohydrolase
-
 Geobacillus stearothermophilus
3.5.4.5 cytidine/2'-deoxycytidine aminohydrolase
-
 [Bacillus] caldolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.5 70 90 the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C Geobacillus stearothermophilus
3.5.4.5 70 90 the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C [Bacillus] caldolyticus