BRENDA - Enzyme Database

Characterization of plant sulfiredoxin and role of sulphinic form of 2-Cys peroxiredoxin

Iglesias-Baena, I.; Barranco-Medina, S.; Lazaro-Payo, A.; Lopez-Jaramillo, F.J.; Sevilla, F.; Lazaro, J.J.; J. Exp. Bot. 61, 1509-1521 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.98.2
DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
1.11.1.15
expression of His-tagged 2-Cys Prx in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C72S
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
E76A
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
K40Q
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
additional information
construction of a Srx knockout mutant, phenotype, overview
Arabidopsis thaliana
1.8.98.2
R28Q
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
R28Q/E76A
site-directed mutagenesis, inactive mutant
Arabidopsis thaliana
1.11.1.15
additional information
construction of a 2-Cys-Prx knockout mutant, phenotype, overview
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.98.2
additional information
-
additional information
kinetic analysis, overview
Arabidopsis thaliana
1.8.98.2
0.029
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate
Arabidopsis thaliana
1.8.98.2
0.079
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.98.2
chloroplast
Srx possesses a chloroplast transit peptide in the N-terminus
Arabidopsis thaliana
9507
-
1.11.1.15
chloroplast
-
Arabidopsis thaliana
9507
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
required
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.98.2
11546
-
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation
Arabidopsis thaliana
1.8.98.2
13914
-
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.2
Arabidopsis thaliana
-
-
-
1.8.98.2
Arabidopsis thaliana Columbia
-
-
-
1.11.1.15
Arabidopsis thaliana
Q96291
-
-
1.11.1.15
Arabidopsis thaliana Columbia
Q96291
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.2
recombinant wild-type Srx and mutants to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
Arabidopsis thaliana
1.11.1.15
recombinant His-tagged 2-Cys Prx from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.98.2
leaf
-
Arabidopsis thaliana
-
1.11.1.15
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
additional information
assay conditions optimization, overview
712607
Arabidopsis thaliana
?
-
-
-
-
1.8.98.2
additional information
assay conditions optimization, overview
712607
Arabidopsis thaliana Columbia
?
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
712607
Arabidopsis thaliana
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
712607
Arabidopsis thaliana Columbia
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
712607
Arabidopsis thaliana
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
712607
Arabidopsis thaliana Columbia
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
712607
Arabidopsis thaliana
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
712607
Arabidopsis thaliana Columbia
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.98.2
?
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation
Arabidopsis thaliana
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.8.98.2
30
-
assay at
Arabidopsis thaliana
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.98.2
0.00042
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate
Arabidopsis thaliana
1.8.98.2
0.011
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.98.2
7.8
-
-
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Arabidopsis thaliana
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.8.98.2
Arabidopsis thaliana
mature enzyme, sequence calculation
-
9.86
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
1.11.1.15
expression of His-tagged 2-Cys Prx in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C72S
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
E76A
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
K40Q
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
additional information
construction of a Srx knockout mutant, phenotype, overview
Arabidopsis thaliana
1.8.98.2
R28Q
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Arabidopsis thaliana
1.8.98.2
R28Q/E76A
site-directed mutagenesis, inactive mutant
Arabidopsis thaliana
1.11.1.15
additional information
construction of a 2-Cys-Prx knockout mutant, phenotype, overview
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.98.2
additional information
-
additional information
kinetic analysis, overview
Arabidopsis thaliana
1.8.98.2
0.029
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate
Arabidopsis thaliana
1.8.98.2
0.079
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.98.2
chloroplast
Srx possesses a chloroplast transit peptide in the N-terminus
Arabidopsis thaliana
9507
-
1.11.1.15
chloroplast
-
Arabidopsis thaliana
9507
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
required
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.98.2
11546
-
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation
Arabidopsis thaliana
1.8.98.2
13914
-
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation
Arabidopsis thaliana
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
recombinant wild-type Srx and mutants to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
Arabidopsis thaliana
1.11.1.15
recombinant His-tagged 2-Cys Prx from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.98.2
leaf
-
Arabidopsis thaliana
-
1.11.1.15
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
additional information
assay conditions optimization, overview
712607
Arabidopsis thaliana
?
-
-
-
-
1.8.98.2
additional information
assay conditions optimization, overview
712607
Arabidopsis thaliana Columbia
?
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
712607
Arabidopsis thaliana
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
712607
Arabidopsis thaliana Columbia
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
712607
Arabidopsis thaliana
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
712607
Arabidopsis thaliana Columbia
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
712607
Arabidopsis thaliana
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
712607
Arabidopsis thaliana Columbia
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.98.2
?
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation
Arabidopsis thaliana
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.8.98.2
30
-
assay at
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.98.2
0.00042
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate
Arabidopsis thaliana
1.8.98.2
0.011
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
pH 7.5, 30C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.98.2
7.8
-
-
Arabidopsis thaliana
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.8.98.2
Arabidopsis thaliana
mature enzyme, sequence calculation
-
9.86
General Information
EC Number
General Information
Commentary
Organism
1.8.98.2
physiological function
the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfinic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx and the conserved Srx is capable of regenerating the functionality of both pea and Arabidopsis Prx-SO2H
Arabidopsis thaliana
1.11.1.15
malfunction
in conditions of oxidative stress, the peroxidatic cysteine can be overoxidized to sulfinic acid inactivating the Prx, the sulfinic 2-Cys Prx is reduced by sulfiredoxin, EC 1.8.98.2, overview
Arabidopsis thaliana
1.11.1.15
additional information
the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfenic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx by sulfiredoxin, catalytic cycle of 2-Cys Prx, overview. The knockout mutant plants without Srx or 2-Cys Prx exhibit phenotypical differences under growth conditions of 16 h light, probably due to the signalling role of the sulfinic form of Prx
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.8.98.2
physiological function
the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfinic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx and the conserved Srx is capable of regenerating the functionality of both pea and Arabidopsis Prx-SO2H
Arabidopsis thaliana
1.11.1.15
malfunction
in conditions of oxidative stress, the peroxidatic cysteine can be overoxidized to sulfinic acid inactivating the Prx, the sulfinic 2-Cys Prx is reduced by sulfiredoxin, EC 1.8.98.2, overview
Arabidopsis thaliana
1.11.1.15
additional information
the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfenic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx by sulfiredoxin, catalytic cycle of 2-Cys Prx, overview. The knockout mutant plants without Srx or 2-Cys Prx exhibit phenotypical differences under growth conditions of 16 h light, probably due to the signalling role of the sulfinic form of Prx
Arabidopsis thaliana