EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.98.2 | DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.98.2 | C72S | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Arabidopsis thaliana |
1.8.98.2 | E76A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Arabidopsis thaliana |
1.8.98.2 | K40Q | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Arabidopsis thaliana |
1.8.98.2 | additional information | construction of a Srx knockout mutant, phenotype, overview | Arabidopsis thaliana |
1.8.98.2 | R28Q | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Arabidopsis thaliana |
1.8.98.2 | R28Q/E76A | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.98.2 | additional information | - |
additional information | kinetic analysis, overview | Arabidopsis thaliana | |
1.8.98.2 | 0.029 | - |
peroxiredoxin-(S-hydroxy-S-oxocysteine) | pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate | Arabidopsis thaliana | |
1.8.98.2 | 0.079 | - |
peroxiredoxin-(S-hydroxy-S-oxocysteine) | pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.98.2 | chloroplast | Srx possesses a chloroplast transit peptide in the N-terminus | Arabidopsis thaliana | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.2 | Mg2+ | required | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.98.2 | 11546 | - |
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation | Arabidopsis thaliana |
1.8.98.2 | 13914 | - |
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.98.2 | Arabidopsis thaliana | - |
- |
- |
1.8.98.2 | Arabidopsis thaliana Columbia | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.98.2 | recombinant wild-type Srx and mutants to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.98.2 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.98.2 | additional information | assay conditions optimization, overview | Arabidopsis thaliana | ? | - |
? | |
1.8.98.2 | additional information | assay conditions optimization, overview | Arabidopsis thaliana Columbia | ? | - |
? | |
1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT | - |
Arabidopsis thaliana | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide | - |
? | |
1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT | - |
Arabidopsis thaliana Columbia | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide | - |
? | |
1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH | - |
Arabidopsis thaliana | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G | - |
? | |
1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH | - |
Arabidopsis thaliana Columbia | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G | - |
? | |
1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin | - |
Arabidopsis thaliana | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide | - |
? | |
1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin | - |
Arabidopsis thaliana Columbia | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.98.2 | ? | x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.98.2 | Srx | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.98.2 | 30 | - |
assay at | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.98.2 | 0.00042 | - |
peroxiredoxin-(S-hydroxy-S-oxocysteine) | pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate | Arabidopsis thaliana | |
1.8.98.2 | 0.011 | - |
peroxiredoxin-(S-hydroxy-S-oxocysteine) | pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.98.2 | 7.8 | - |
- |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.2 | ATP | - |
Arabidopsis thaliana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.8.98.2 | Arabidopsis thaliana | mature enzyme, sequence calculation | - |
9.86 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.98.2 | physiological function | the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfinic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx and the conserved Srx is capable of regenerating the functionality of both pea and Arabidopsis Prx-SO2H | Arabidopsis thaliana |