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Literature summary extracted from

  • Iglesias-Baena, I.; Barranco-Medina, S.; Lazaro-Payo, A.; Lopez-Jaramillo, F.J.; Sevilla, F.; Lazaro, J.J.
    Characterization of plant sulfiredoxin and role of sulphinic form of 2-Cys peroxiredoxin (2010), J. Exp. Bot., 61, 1509-1521.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.98.2 DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.8.98.2 C72S site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Arabidopsis thaliana
1.8.98.2 E76A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Arabidopsis thaliana
1.8.98.2 K40Q site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Arabidopsis thaliana
1.8.98.2 additional information construction of a Srx knockout mutant, phenotype, overview Arabidopsis thaliana
1.8.98.2 R28Q site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Arabidopsis thaliana
1.8.98.2 R28Q/E76A site-directed mutagenesis, inactive mutant Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.98.2 additional information
-
additional information kinetic analysis, overview Arabidopsis thaliana
1.8.98.2 0.029
-
peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate Arabidopsis thaliana
1.8.98.2 0.079
-
peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.98.2 chloroplast Srx possesses a chloroplast transit peptide in the N-terminus Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.98.2 Mg2+ required Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.98.2 11546
-
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation Arabidopsis thaliana
1.8.98.2 13914
-
x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.8.98.2 Arabidopsis thaliana
-
-
-
1.8.98.2 Arabidopsis thaliana Columbia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.2 recombinant wild-type Srx and mutants to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.98.2 leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.2 additional information assay conditions optimization, overview Arabidopsis thaliana ?
-
?
1.8.98.2 additional information assay conditions optimization, overview Arabidopsis thaliana Columbia ?
-
?
1.8.98.2 peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
Arabidopsis thaliana peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
?
1.8.98.2 peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 DTT
-
Arabidopsis thaliana Columbia peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + DTT disulfide
-
?
1.8.98.2 peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
Arabidopsis thaliana peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
?
1.8.98.2 peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 GSH
-
Arabidopsis thaliana Columbia peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + G-S-S-G
-
?
1.8.98.2 peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
Arabidopsis thaliana peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
?
1.8.98.2 peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 thioredoxin
-
Arabidopsis thaliana Columbia peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + thioredoxin disulfide
-
?

Subunits

EC Number Subunits Comment Organism
1.8.98.2 ? x * 13914, immature enzyme, sequence calculation, x * 11546, mature enzyme, sequence calculation Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.8.98.2 Srx
-
Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.98.2 30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.98.2 0.00042
-
peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate Arabidopsis thaliana
1.8.98.2 0.011
-
peroxiredoxin-(S-hydroxy-S-oxocysteine) pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.98.2 7.8
-
-
Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.98.2 ATP
-
Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.98.2 Arabidopsis thaliana mature enzyme, sequence calculation
-
9.86

General Information

EC Number General Information Comment Organism
1.8.98.2 physiological function the antioxidant function of 2-Cys peroxiredoxin, Prx, EC 1.11.1.15, involves the oxidation of its conserved peroxidatic cysteine to sulfinic acid that is recycled by a reductor agent. Sulfiredoxin reduces the sulfinic 2-Cys Prx, Prx-SO2H. The activity of sulfiredoxin is dependent on the concentration of the sulfinic form of Prx and the conserved Srx is capable of regenerating the functionality of both pea and Arabidopsis Prx-SO2H Arabidopsis thaliana