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Literature summary extracted from

  • Xu, D.Y.; Yang, Y.; Yang, Z.
    Activity and stability of cross-linked tyrosinase aggregates in aqueous and nonaqueous media (2011), J. Biotechnol., 152, 30-36.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 1,3-dimethylimidazolium methylsulfate 69.7% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 1-butyl-3-methylimidazolium methylsulfate 47.8% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 1-ethyl-3-methylimidazolium methylsulfate 64.1% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 acetone increasing solvent concentration up to 80% (v/v) yields a gradual reduction in the activity of the soluble and cross-linked enzyme forms, the cross-linked enzyme aggregate shows about 40% residual activity after incubation in acetone for about 34 h Agaricus bisporus
1.14.18.1 chloroform the cross-linked enzyme aggregate shows about 30% residual activity after incubation in chloroform for about 3 h Agaricus bisporus
1.14.18.1 choline acetate 27.9% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 choline dihydrophosphate 27.4% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 choline methylsulfonate 39.7% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 choline nitrate 54.6% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 dioxane increasing solvent concentration up to 80% (v/v) yields a gradual reduction in the activity of the soluble and cross-linked enzyme forms, the cross-linked enzyme aggregate shows about 40% residual activity after incubation in dioxane for about 62 h Agaricus bisporus
1.14.18.1 EDTA
-
 Agaricus bisporus
1.14.18.1 Guanidine-HCl treatment with guanidine-HCl at increasing concentrations (0-800 mM) results in a reduced activity for both enzyme forms, but aggregation as cross-linked enzyme aggregate improves tyrosinase stability at higher concentrations (above 314 mM) Agaricus bisporus
1.14.18.1 hexane the cross-linked enzyme aggregate shows about 20% residual activity after incubation in hexane for about 24 h Agaricus bisporus
1.14.18.1 methanol the soluble enzyme retains 7.8% of its original activity, as compared to 31% by the cross-linked enzyme aggregates, after being incubated in the presence of 40% (v/v) methanol Agaricus bisporus
1.14.18.1 NaN3 treatment with NaN3 at increasing concentrations (0-4 mM) results in a reduced activity for both soluble and cross-linked enzyme forms, but aggregation as cross-linked enzyme aggregates improves tyrosinase stability at higher concentrations (above 0.4 mM) Agaricus bisporus
1.14.18.1 SDS a sharp decrease in the activity of the soluble enzyme is noted from 0 to 17 mM SDS Agaricus bisporus
1.14.18.1 tert-butanol the cross-linked enzyme aggregate shows about 50% residual activity after incubation in tert-butanol for about 326 h Agaricus bisporus
1.14.18.1 tetrabutylammonium acetate 24.1% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 tetrabutylammonium methylsulfonate 45.3% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 tetramethylammonium acetate 30% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 tributylammonium dihydrophosphate 27.5% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 triethylammonium dihydrophosphate 23.4% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 trimethylammonium dihydrophosphate 12% residual activity at 5% (w/v) Agaricus bisporus
1.14.18.1 trimethylammonium methylsulfonate 40.9% residual activity at 5% (w/v) Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.41
-
 L-Dopa soluble enzyme, pH 6.0, 55°C Agaricus bisporus
1.14.18.1 1.16
-
 L-Dopa cross-linked enzyme aggregate, pH 6.0, 30°C Agaricus bisporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ the enzyme contains copper Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-DOPA + O2
-
 Agaricus bisporus dopaquinone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyrosinase
-
 Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 30 40 cross-linked enzyme aggregate Agaricus bisporus
1.14.18.1 55
-
 soluble enzyme Agaricus bisporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 40 60 the soluble enzyme shows half-lives of about 120 min, 30 min, and 10 min at 40, 50, and 60°C, respectively. The cross-linked enzyme aggregates show half-lives of about 320 min, 120 min, and 30 min at 40, 50, and 60°C, respectively Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6
-
-
 Agaricus bisporus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 5 8 the soluble enzyme shows half-lives of about 11 min, 35 min, and 3 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively. The cross-linked enzyme aggregates show half-lives of about 11 min, 35 min, and 1 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively Agaricus bisporus