EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.27 | expressed in Escherichia coli strain K12 JM109 | Bacillus thermoproteolyticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.27 | D150E | the mutation increases the activity of thermolysin | Bacillus thermoproteolyticus |
3.4.24.27 | G8C/N60C/S65P | the mutation increases the stability of thermolysin | Bacillus thermoproteolyticus |
3.4.24.27 | G8C/N60C/S65P | the triple mutation increases the stability of thermolysin as high as the individual mutations do | Bacillus thermoproteolyticus |
3.4.24.27 | G8C/N60C/S65P/L144S | the mutant is more active and stable than wild type thermolysin | Bacillus thermoproteolyticus |
3.4.24.27 | I168A | the mutation increases the activity of thermolysin and shows about 90% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | L144S | the mutation increases the activity of thermolysin and shows about 30% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | L144S/D150E | the mutation yields the most significant increase in the hydrolytic activities for N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide and N-carbobenzoxy-L-Asp-L-Phe methyl ester and shows about 30% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | L144S/D150E/I168A/S53D/L155A/G8C/N60C/S65P | inactive | Bacillus thermoproteolyticus |
3.4.24.27 | L144S/D150E/L155A | inactive | Bacillus thermoproteolyticus |
3.4.24.27 | L144S/D150E/S53D | the triple mutant shows improved activity and stability with about 30% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | L144S/D150E/S53D/L155A | inactive | Bacillus thermoproteolyticus |
3.4.24.27 | L144S/I168A | the mutation abolishes the hydrolytic activities for N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide and N-carbobenzoxy-L-Asp-L-Phe methyl ester | Bacillus thermoproteolyticus |
3.4.24.27 | L155A | the mutation increases the stability of thermolysin and shows about 60% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | L155A/G8C/N60C/S65P | the mutant shows about 80% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | S53D | the mutation increases the stability of thermolysin | Bacillus thermoproteolyticus |
3.4.24.27 | S53D/G8C/N60C/S65P | the mutant shows about 110% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | S53D/L155A | the mutation yields the greatest increase in the thermal stability and shows about 90% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
3.4.24.27 | S53D/L155A/G8C/N60C/S65P | the mutant shows about 70% casein-hydrolytic activity compared to the wild type enzyme | Bacillus thermoproteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.16 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.17 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.18 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme D150E/I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.19 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.46 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.5 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.54 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.55 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.63 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | wild type enzyme, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.69 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.77 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.83 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.94 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 1.29 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.27 | Na+ | 4 M Na+ stimulates the hydrolytic activity of wild type thermolysin about 13fold | Bacillus thermoproteolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.27 | Bacillus thermoproteolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.27 | - |
Bacillus thermoproteolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.27 | 3.5 | - |
purified mutant enzyme L144S/D150E, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 3.6 | - |
purified mutant enzyme L144S, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 6.7 | - |
purified mutant enzyme L155A, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 7.5 | - |
purified mutant enzyme D150E/I168A, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 7.9 | - |
purified mutant enzyme S53D/L155A/G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 8.1 | - |
purified mutant enzyme L155A/G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 9.6 | - |
purified mutant enzyme I168A, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 10.2 | - |
purified mutant enzyme S53D/L155A, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 10.5 | - |
purified mutant enzyme D150E, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 10.8 | - |
purified mutant enzyme G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 10.8 | - |
purified wild type enzyme, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 11.3 | - |
purified mutant enzyme S53D, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
3.4.24.27 | 11.9 | - |
purified mutant enzyme S53D/G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C | Bacillus thermoproteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.27 | casein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
3.4.24.27 | N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
3.4.24.27 | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.27 | 80 | - |
the rate constant (kobs) for thermal inactivation at 80°C is 84000 s-1 for the wild type enzyme | Bacillus thermoproteolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 3.3 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 5.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 5.9 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | wild type enzyme, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6.3 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6.9 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 7.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 7.7 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 8.7 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 9.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 15.5 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme D150E/I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 15.6 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 16.5 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 17 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.23 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D/L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.26 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.29 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.29 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | wild type enzyme, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.31 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.32 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.33 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D/L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.34 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.62 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme I168A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.79 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 1.82 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L144S, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 2.04 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L144S/D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 2.1 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 2.49 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L144S/D150E/S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 3 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme I168A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 3.2 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D/L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 3.3 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D/L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 3.5 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 3.8 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | wild type enzyme, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 3.9 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 4 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 4 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 4.3 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L144S/D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6.6 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L144S, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 6.7 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 7.5 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 7.9 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 9 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 9.2 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 9.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | wild type enzyme, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 10 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 11.7 | - |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | mutant enzyme L144S/D150E/S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 16.8 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 33.1 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 49.7 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 96.2 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus | |
3.4.24.27 | 99 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C | Bacillus thermoproteolyticus |