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Literature summary extracted from

  • Bhave, D.P.; Hong, J.A.; Lee, M.; Jang, W.; Krebs, C.; Carroll, K.S.
    Spectroscopic studies on the [4Fe-4S] cluster in adenosine 5-phosphosulfate reductase from Mycobacterium tuberculosis (2011), J. Biol. Chem., 286, 1216-1226.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.8.4.8 medicine in Mycobacterium tuberculosis, APR is a validated target against the latent phase of infection Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.8 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.8 K144A | the mutation causes a 63000fold increase of kcat/KM and decreases the value of kmax by 270fold. Relative to the wild type enzyme, the affinity of adenosine 5’-phosphosulfate for K144A is decreased by 400fold Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.8 Fe iron-sulfur protein, each mole of protein contains 3.3 mol of Fe Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.8 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.8 HiTrap chelating column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.8 adenosine 5'-phosphosulfate + thioredoxin
-
 Mycobacterium tuberculosis AMP + sulfite + oxidized thioredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.8 adenosine 5'-phosphosulfate reductase
-
 Mycobacterium tuberculosis
1.8.4.8 APR
-
 Mycobacterium tuberculosis
1.8.4.8 APS reductase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.8 4Fe-4S-center the enzyme harbors a [4Fe-4S]2+ cluster Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.8 0.04
-
 adenosine 5'-phosphosulfate mutant enzyme K144A, at 30°C in 100 mM bis-Tris propane buffer, pH 7.5 Mycobacterium tuberculosis
1.8.4.8 2500
-
 adenosine 5'-phosphosulfate wild type enzyme, at 30°C in 100 mM bis-Tris propane buffer, pH 7.5 Mycobacterium tuberculosis