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Literature summary extracted from

  • Hu, W.; Xu, R.; Sun, W.; Szulc, Z.M.; Bielawski, J.; Obeid, L.M.; Mao, C.
    Alkaline ceramidase 3 (ACER3) hydrolyzes unsaturated long-chain ceramides, and its down-regulation inhibits both cell proliferation and apoptosis (2010), J. Biol. Chem., 285, 7964-7976.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 transient overexpression of FLAG-tagged ACER3 in HSC-1 cells Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 ceramide + H2O Homo sapiens
-
 sphingosine + fatty acid
-
 ?
3.5.1.23 dihydroceramide + H2O Homo sapiens
-
 dihydrosphingosine + fatty acid
-
 ?
3.5.1.23 phytoceramide + H2O Homo sapiens
-
 phytosphingosine + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 11-eicosenoyl-ceramide + H2O
-
 Homo sapiens sphingosine + 11-eicosenoic acid
-
 ?
3.5.1.23 11-eicosenoyl-dihydroceramide + H2O
-
 Homo sapiens dihydrosphingosine + 11-eicosenoic acid
-
 ?
3.5.1.23 11-eicosenoyl-phytoceramide + H2O
-
 Homo sapiens phytosphingosine + 11-eicosenoic acid
-
 ?
3.5.1.23 ceramide + H2O
-
 Homo sapiens sphingosine + fatty acid
-
 ?
3.5.1.23 dihydroceramide + H2O
-
 Homo sapiens dihydrosphingosine + fatty acid
-
 ?
3.5.1.23 additional information ACER3 hydrolyzes C18:1-, C20:1-, C20:4-ceramides, C18:1-, C20:1-, C20:4-dihydroceramides, and C18:1-, C20:1-, C20:4-phytoceramides in vitro Homo sapiens ?
-
 ?
3.5.1.23 N-arachidonoyl-ceramide + H2O
-
 Homo sapiens sphingosine + arachidonate
-
 ?
3.5.1.23 N-arachidonoyl-dihydroceramide + H2O
-
 Homo sapiens dihydrosphingosine + arachidonate
-
 ?
3.5.1.23 N-arachidonoyl-phytoceramide + H2O
-
 Homo sapiens phytosphingosine + arachidonate
-
 ?
3.5.1.23 N-oleoyl-ceramide + H2O
-
 Homo sapiens sphingosine + oleate
-
 ?
3.5.1.23 N-oleoyl-dihydroceramide + H2O
-
 Homo sapiens dihydrosphingosine + oleate
-
 ?
3.5.1.23 N-oleoyl-phytoceramide + H2O
-
 Homo sapiens phytosphingosine + oleate
-
 ?
3.5.1.23 phytoceramide + H2O
-
 Homo sapiens phytosphingosine + fatty acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 ACER2
-
 Homo sapiens
3.5.1.23 ACER3
-
 Homo sapiens
3.5.1.23 alkaline ceramidase 2
-
 Homo sapiens
3.5.1.23 alkaline ceramidase 3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 9
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.1.23 additional information in cells, ACER3 overexpression decreases C18:1- and C20:1-ceramides and dihydroceramides, whereas ACER3 knockdown by RNA interference in HeLa cells has the opposite effect. ACER3 knockdown inhibits cell proliferation and up-regulates the cyclin-dependent kinase inhibitor p21CIP1/WAF1. Blocking p21CIP1/WAF1 up-regulation attenuates the inhibitory effect of ACER3 knockdown on cell proliferation, suggesting that ACER3 knockdown inhibits cell proliferation because of p21CIP1/WAF1 up-regulation. ACER3 knockdown inhibits cell apoptosis in response to serum deprivation. ACER3 knockdown up-regulates the expression of ACER2, and the ACER2 up-regulation decreases non-ULC ceramide species while increasing both sphingosine and its phosphate Homo sapiens
3.5.1.23 physiological function ACER3 specifically controls the hydrolysis of ceramides carrying unsaturated long acyl chains, unsaturated long-chain ceramides Homo sapiens