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Literature summary extracted from

  • Gantt, S.L.; Joseph, C.G.; Fierke, C.A.
    Activation and inhibition of histone deacetylase 8 by monovalent cations (2010), J. Biol. Chem., 285, 6036-6043.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.98
-
Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.98 D176A the mutation increases the K1/2 for potassium inhibition by more than 40fold Homo sapiens
3.5.1.98 H142A the mutation increases the K1/2 for potassium inhibition by more than 40fold Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.98 K+ K+ bound to monovalent cation site 1 inhibits catalytic activity of HDAC8 (11fold less active with two K+ ions bound compared to one K+ ion bound), partial inhibition at high KCl Homo sapiens
3.5.1.98 suberoylanilide hydroxamic acid
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.98 K+ K+ bound to monovalent cation site 2 enhances catalytic activity of HDAC8 45fold with maximal deacetylase activity observed at 10 mM KCl. K+ is the predominant monovalent cation bound to HDAC8 in vivo, K+ binding to site 1 enhances the affinity of HDAC8 for suberoylanilide hydroxamic acid Homo sapiens
3.5.1.98 Na+ Na+ binds more weakly to both monovalent cation sites and activates HDAC8 to a lesser extent than K+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.98 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.1.98 Co(2+)-HDAC8 is stable in the presence of oxygen Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.98
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.98 Fluor de Lys H4-AcK16 + H2O
-
Homo sapiens ?
-
?
3.5.1.98 Fluor de Lys HDAC8 substrate + H2O
-
Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.98 HDAC8
-
Homo sapiens
3.5.1.98 histone deacetylase 8
-
Homo sapiens