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Literature summary extracted from

  • Chiu, F.Y.; Chen, Y.R.; Tu, S.L.
    Electrostatic interaction of phytochromobilin synthase and ferredoxin for biosynthesis of phytochrome chromophore (2010), J. Biol. Chem., 285, 5056-5065.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.4 E110Q site-directed mutagenesis, the mutant shows 321.7% of wild-type activity Arabidopsis thaliana
1.3.7.4 E187Q site-directed mutagenesis, the mutant shows 20.3% of wild-type activity Arabidopsis thaliana
1.3.7.4 H259Q site-directed mutagenesis, the mutant shows 123.4% of wild-type activity Arabidopsis thaliana
1.3.7.4 K183Q site-directed mutagenesis, the mutant shows 24.6% of wild-type activity Arabidopsis thaliana
1.3.7.4 K255Q site-directed mutagenesis, the mutant shows 11.7% of wild-type activity Arabidopsis thaliana
1.3.7.4 K263Q site-directed mutagenesis, the mutant shows 25.8% of wild-type activity Arabidopsis thaliana
1.3.7.4 R200Q site-directed mutagenesis, the mutant shows 12.5% of wild-type activity Arabidopsis thaliana
1.3.7.4 R200Q/R264Q site-directed mutagenesis, the mutant shows 11.9% of wild-type activity Arabidopsis thaliana
1.3.7.4 R264Q site-directed mutagenesis, the mutant shows 18.9% of wild-type activity Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.7.4 Fe2+ cofactor ferredoxin Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.4 biliverdin IXalpha + reduced ferredoxin Arabidopsis thaliana
-
 (3Z)-phytochromobilin + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.4 Arabidopsis thaliana Q9SR43
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.4 biliverdin IXalpha + reduced ferredoxin
-
 Arabidopsis thaliana (3Z)-phytochromobilin + oxidized ferredoxin
-
 ?
1.3.7.4 biliverdin IXalpha + reduced ferredoxin conserved surface-charged residues on HY2 and Arabidopsis ferredoxin AtFd2 are important in the protein-protein interaction as well as biliverdin reduction activity of HY2, mutational analysis, overview. The C12 propionate group of biliverdin is important for HY2-catalyzed biliverdin reduction Arabidopsis thaliana (3Z)-phytochromobilin + oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.4 HY2
-
 Arabidopsis thaliana
1.3.7.4 phytochromobilin synthase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.7.4 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.7.4 8.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.4 Ferredoxin one of the six Arabidopsis ferredoxins, AtFd2, is the preferred electron donor for HY2. Activity with ferredoxin AtFd2 mutants D78N, E81Q, E82Q, Y89F, R92Q, and D112N, overview Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.3.7.4 physiological function HY2 synthesizes the open chain tetrapyrrole chromophore for light-sensing phytochromes. It catalyzes the double bond reduction of a heme-derived tetrapyrrole intermediate biliverdin IXalpha at the A-ring diene system Arabidopsis thaliana