EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.7.4 | E110Q | site-directed mutagenesis, the mutant shows 321.7% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | E187Q | site-directed mutagenesis, the mutant shows 20.3% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | H259Q | site-directed mutagenesis, the mutant shows 123.4% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | K183Q | site-directed mutagenesis, the mutant shows 24.6% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | K255Q | site-directed mutagenesis, the mutant shows 11.7% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | K263Q | site-directed mutagenesis, the mutant shows 25.8% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | R200Q | site-directed mutagenesis, the mutant shows 12.5% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | R200Q/R264Q | site-directed mutagenesis, the mutant shows 11.9% of wild-type activity | Arabidopsis thaliana |
1.3.7.4 | R264Q | site-directed mutagenesis, the mutant shows 18.9% of wild-type activity | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.4 | Fe2+ | cofactor ferredoxin | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.4 | biliverdin IXalpha + reduced ferredoxin | Arabidopsis thaliana | - |
(3Z)-phytochromobilin + oxidized ferredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.7.4 | Arabidopsis thaliana | Q9SR43 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.4 | biliverdin IXalpha + reduced ferredoxin | - |
Arabidopsis thaliana | (3Z)-phytochromobilin + oxidized ferredoxin | - |
? | |
1.3.7.4 | biliverdin IXalpha + reduced ferredoxin | conserved surface-charged residues on HY2 and Arabidopsis ferredoxin AtFd2 are important in the protein-protein interaction as well as biliverdin reduction activity of HY2, mutational analysis, overview. The C12 propionate group of biliverdin is important for HY2-catalyzed biliverdin reduction | Arabidopsis thaliana | (3Z)-phytochromobilin + oxidized ferredoxin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.7.4 | HY2 | - |
Arabidopsis thaliana |
1.3.7.4 | phytochromobilin synthase | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.7.4 | 25 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.7.4 | 8.5 | - |
assay at | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.4 | Ferredoxin | one of the six Arabidopsis ferredoxins, AtFd2, is the preferred electron donor for HY2. Activity with ferredoxin AtFd2 mutants D78N, E81Q, E82Q, Y89F, R92Q, and D112N, overview | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.7.4 | physiological function | HY2 synthesizes the open chain tetrapyrrole chromophore for light-sensing phytochromes. It catalyzes the double bond reduction of a heme-derived tetrapyrrole intermediate biliverdin IXalpha at the A-ring diene system | Arabidopsis thaliana |