EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.3.2.1 | Selenite | competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.1 | 0.02 | - |
phosphate | pH 6.0, 30°C, Vmax: 860 pmol of phosphate incorporated per min/OD650 | Saccharomyces cerevisiae | |
7.3.2.1 | 0.025 | - |
phosphate | using triple mutant DELTApho87-DELTApho90-DELTApho91, pH 6.0, 30°C, Vmax: 2000 pmol of phosphate incorporated per min/OD650 | Saccharomyces cerevisiae | |
7.3.2.1 | 0.025 | - |
phosphate | wild-type, pH 6.0, 30°C, Vmax: 440 pmol of phosphate incorporated per min/OD650 | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.3.2.1 | Saccharomyces cerevisiae | P25297 | - |
- |
7.3.2.1 | Saccharomyces cerevisiae | P25360 | - |
- |
7.3.2.1 | Saccharomyces cerevisiae | P27514 | - |
- |
7.3.2.1 | Saccharomyces cerevisiae | P39535 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.1 | ATP + H2O + phosphate/out | - |
Saccharomyces cerevisiae | ADP + phosphate + phosphate/in | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.3.2.1 | high-affinity phosphate transporter Pho84p | - |
Saccharomyces cerevisiae |
7.3.2.1 | low-affinity phosphate transporter Pho87p | - |
Saccharomyces cerevisiae |
7.3.2.1 | low-affinity phosphate transporter Pho90p | - |
Saccharomyces cerevisiae |
7.3.2.1 | low-affinity phosphate transporter Pho91p | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.3.2.1 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.3.2.1 | 6 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.1 | 4.6 | - |
Selenite | - |
Saccharomyces cerevisiae | |
7.3.2.1 | 5.5 | - |
Selenite | using triple mutant DELTApho87-DELTApho90-DELTApho91 | Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.3.2.1 | malfunction | a triple mutant consisting of deleted low-affinity transporters Pho87, Pho90 and Pho91 is slightly more sensitive to selenite than the wild-type strain. Single mutants do not show any difference to the wild-type stain | Saccharomyces cerevisiae |
7.3.2.1 | malfunction | a triple mutant consisting of deleted low-affinity transporters Pho87, Pho90 and Pho91 is slightly more sensitive to selenite than the wild-type strain. Single mutants do not show any difference to the wild-type strain | Saccharomyces cerevisiae |
7.3.2.1 | malfunction | selenite uptake is reduced in a DELTA pho84 mutant | Saccharomyces cerevisiae |
7.3.2.1 | physiological function | when cells are grown at low phosphate concentrations, the high-affinity phosphate transporter Pho84p is the major contributor to selenite uptake. Pho84p is very selective for phosphate as compared to selenite | Saccharomyces cerevisiae |
7.3.2.1 | physiological function | when phosphate is abundant, selenite is internalized through the low-affinity phosphate transporters (Pho87p, Pho90p, and Pho91p). Low-affinity transporters discriminate less efficiently between selenite and phosphate | Saccharomyces cerevisiae |