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Literature summary extracted from

  • Gardner, A.M.; Cook, M.R.; Gardner, P.R.
    Nitric-oxide dioxygenase function of human cytoglobin with cellular reductants and in rat hepatocytes (2010), J. Biol. Chem., 285, 23850-23857.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.12.17 ascorbate stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 1000 M/s, Km is 0.25 mM Homo sapiens
1.14.12.17 cytochrome b5 stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 3 x 1000000 M/s, Km is 0.3 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.17 functional expression of human cytoglobin in rat hepatocytes and effects on the cell NO metabolism, overview. Expression in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.17 CO a competitive inhibitor Homo sapiens
1.14.12.17 cyanide causes slow inactivation Homo sapiens
1.14.12.17 additional information anions competitively inhibit activity. Increasing the sodium phosphate concentration or including sodium phosphate or NaCl in a 25 mM sodium citrate buffer also competitively inhibits the activity and increases the Km value of ascorbate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.17 additional information
-
 additional information Michaelis-Menten behavior of the Cygb-NOD activity with ascorbate Homo sapiens
1.14.12.17 0.00002
-
 O2 pH 7.0, 37°C Homo sapiens
1.14.12.17 0.00004
-
 nitric oxide pH 7.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.17 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.12.17 hepatocyte
-
 Homo sapiens
-
1.14.12.17 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.17 2 nitric oxide + 2 O2 + NADPH via an Cygb-NOD intermediate, putative ascorbate-binding site in Cygb, overview Homo sapiens 2 nitrate + NADP+ + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.17 Cygb
-
 Homo sapiens
1.14.12.17 cytoglobin
-
 Homo sapiens
1.14.12.17 nitric-oxide dioxygenase
-
 Homo sapiens
1.14.12.17 NO dioxygenase
-
 Homo sapiens
1.14.12.17 NOD
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.17 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.17 NADPH
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.12.17 additional information the enzyme is distinct from the microsomal NOD activity Homo sapiens