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Literature summary extracted from

  • Saga, G.; Giorgetti, A.; Fufezan, C.; Giacometti, G.M.; Bassi, R.; Morosinotto, T.
    Mutation analysis of violaxanthin de-epoxidase identifies substrate-binding sites and residues involved in catalysis (2010), J. Biol. Chem., 285, 23763-23770.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.23.5.1 expressed in Escherichia coli Origami B cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.23.5.1 D114A inactive Arabidopsis thaliana
1.23.5.1 D114N inactive Arabidopsis thaliana
1.23.5.1 D177A inactive Arabidopsis thaliana
1.23.5.1 D177N inactive Arabidopsis thaliana
1.23.5.1 D178A the mutant shows 56% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 F123A the mutant shows 34% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 F155A the mutant shows 5% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 H121A the mutant shows 5% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 N167A the mutant shows 121% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 Q119A the mutant shows 32% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 Q153A the mutant shows 75% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 Q153E the mutant shows 60% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 Q153L the mutant shows 30% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 T245A the mutant shows 82% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 W179A the mutant shows less than 2% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 W179N the mutant shows less than 2% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 Y175F the mutant shows 62% activity compared to the wild type enzyme Arabidopsis thaliana
1.23.5.1 Y198F inactive Arabidopsis thaliana
1.23.5.1 Y214F the mutant shows less than 2% activity compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.23.5.1 pepstatin A
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.23.5.1 thylakoid membrane
-
 Arabidopsis thaliana 42651
-

Organism

EC Number Organism UniProt Comment Textmining
1.23.5.1 Arabidopsis thaliana Q39249
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.23.5.1 nickel affinity column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.23.5.1 antheraxanthin + ascorbate
-
 Arabidopsis thaliana zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate
-
 Arabidopsis thaliana antheraxanthin + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.23.5.1 VDE
-
 Arabidopsis thaliana