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Literature summary extracted from

  • Horta, B.B.; de Oliveira, M.A.; Discola, K.F.; Cussiol, J.R.; Netto, L.E.
    Structural and biochemical characterization of peroxiredoxin Qbeta from Xylella fastidiosa: catalytic mechanism and high reactivity (2010), J. Biol. Chem., 285, 16051-16065.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 gene prxq, expression of wild-type and mutant PrxQs in Escherichia coli strain BL21(DE3) Xylella fastidiosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.24 purified recombinant PrxQ C47S, sitting drop vapor diffusion method, 5-15 mg/ml protein in 5 mM sodium citrate, pH 5.0, and 10 mM DTT, are mixed with reservoir solution containing 0.1 M HEPES-HCl, pH 7.5, and 20% PEG 8000 at 20°C, X-ray diffraction structure determination and analysis Xylella fastidiosa

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C101S site-directed mutagenesis, structure comparison to the wild-type enzyme Xylella fastidiosa
1.11.1.24 C23S site-directed mutagenesis, structure comparison to the wild-type enzyme Xylella fastidiosa
1.11.1.24 C47S site-directed mutagenesis, structure comparison to the wild-type enzyme Xylella fastidiosa
1.11.1.24 C83S site-directed mutagenesis, structure comparison to the wild-type enzyme, dimedone likely inactivated the XfPrxQ C83S protein because sulfenic acids persist long enough to react with dimedone only in the absence of Cys83 Xylella fastidiosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.033
-
 H2O2 pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.11.1.24 0.037
-
 cumene hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.11.1.24 0.072
-
 t-butyl hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 17813
-
 x * 17813, sequence calculation Xylella fastidiosa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Xylella fastidiosa Q9PER7 gene prxq or bcp
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.24 0.67
-
 pH 7.4, 37°C, recombinant enzyme, substarte t-butyl hydroperoxide Xylella fastidiosa
1.11.1.24 1.49
-
 pH 7.4, 37°C, recombinant enzyme, substrate cumene hydroperoxide Xylella fastidiosa
1.11.1.24 1.76
-
 pH 7.4, 37°C, recombinant enzyme, substrate H2O2 Xylella fastidiosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 2 thioredoxin + cumene hydroperoxide
-
 Xylella fastidiosa thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
 ?
1.11.1.24 2 thioredoxin + H2O2
-
 Xylella fastidiosa thioredoxin disulfide + 2 H2O
-
 ?
1.11.1.24 2 thioredoxin + t-butyl hydroperoxide
-
 Xylella fastidiosa thioredoxin disulfide + H2O + t-butanol
-
 ?
1.11.1.24 additional information Cys83 is the resolving cysteine of XfPrxQ Xylella fastidiosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 ? x * 17813, sequence calculation Xylella fastidiosa
1.11.1.24 More primary structure and peptide mapping for detection of the disulfide-containing peptide, using chemical modification and tryptic digestion with HPLC-ESI-MS analyses, overview. Conformational analysis of XfPrxQ, modelling, overview Xylella fastidiosa

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 bacterioferritin comigratory protein
-
 Xylella fastidiosa
1.11.1.24 BCP
-
 Xylella fastidiosa
1.11.1.24 More peroxiredoxin PrxQ belongs to the subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins Xylella fastidiosa
1.11.1.24 PrxQ
-
 Xylella fastidiosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.24 37
-
 assay at Xylella fastidiosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 0.41
-
 cumene hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.11.1.24 1.1
-
 H2O2 pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.11.1.24 2
-
 t-butyl hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 7.4
-
 assay at Xylella fastidiosa

General Information

EC Number General Information Comment Organism
1.11.1.24 physiological function to survive under oxidative stress imposed by the host, microorganisms express antioxidant proteins, including cysteine-based peroxidases named peroxiredoxins Xylella fastidiosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.24 1.49
-
 cumene hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.11.1.24 33.5
-
 H2O2 pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.11.1.24 53.4
-
 t-butyl hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa