BRENDA - Enzyme Database show

A bisallylic mini-lipoxygenase from cyanobacterium Cyanothece sp. that has an iron as cofactor

Andreou, A.; Goebel, C.; Hamberg, M.; Feussner, I.; J. Biol. Chem. 285, 14178-14186 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.33
expressed in Escherichia coli BL21 Star cells
Cyanothece sp. ATCC 51142
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.33
Fe2+
LOX2 contains iron with an iron to protein ratio of 45%
Cyanothece sp. ATCC 51142
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.33
65000
-
SDS-PAGE
Cyanothece sp. ATCC 51142
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.33
Cyanothece sp. ATCC 51142
B7JX99
-
-
1.13.11.33
Cyanothece sp. ATCC 51142 PCC 8801
B7JX99
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.33
HisTrap column chromatography, gel filtration
Cyanothece sp. ATCC 51142
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.33
1,2-diarachidonoylglycerophosphocholine + O2
-
712421
Cyanothece sp. ATCC 51142
?
-
-
-
?
1.13.11.33
1,2-diarachidonoylglycerophosphocholine + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
?
-
-
-
?
1.13.11.33
arachidonic acid + O2
-
712421
Cyanothece sp. ATCC 51142
13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
-
-
?
1.13.11.33
arachidonic acid + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
-
-
?
1.13.11.33
linoleate + O2
-
712421
Cyanothece sp. ATCC 51142
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product
-
-
?
1.13.11.33
linoleate + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product
-
-
?
1.13.11.33
linolenate + O2
-
712421
Cyanothece sp. ATCC 51142
11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.33
linolenate + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.33
additional information
incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase
712421
Cyanothece sp. ATCC 51142
?
-
-
-
-
1.13.11.33
additional information
incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase
712421
Cyanothece sp. ATCC 51142 PCC 8801
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.33
9.3
-
-
Cyanothece sp. ATCC 51142
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.33
6
10
-
Cyanothece sp. ATCC 51142
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.33
expressed in Escherichia coli BL21 Star cells
Cyanothece sp. ATCC 51142
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.33
Fe2+
LOX2 contains iron with an iron to protein ratio of 45%
Cyanothece sp. ATCC 51142
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.33
65000
-
SDS-PAGE
Cyanothece sp. ATCC 51142
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.33
HisTrap column chromatography, gel filtration
Cyanothece sp. ATCC 51142
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.33
1,2-diarachidonoylglycerophosphocholine + O2
-
712421
Cyanothece sp. ATCC 51142
?
-
-
-
?
1.13.11.33
1,2-diarachidonoylglycerophosphocholine + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
?
-
-
-
?
1.13.11.33
arachidonic acid + O2
-
712421
Cyanothece sp. ATCC 51142
13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
-
-
?
1.13.11.33
arachidonic acid + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
-
-
?
1.13.11.33
linoleate + O2
-
712421
Cyanothece sp. ATCC 51142
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product
-
-
?
1.13.11.33
linoleate + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate
(9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product
-
-
?
1.13.11.33
linolenate + O2
-
712421
Cyanothece sp. ATCC 51142
11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.33
linolenate + O2
-
712421
Cyanothece sp. ATCC 51142 PCC 8801
11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.33
additional information
incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase
712421
Cyanothece sp. ATCC 51142
?
-
-
-
-
1.13.11.33
additional information
incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase
712421
Cyanothece sp. ATCC 51142 PCC 8801
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.33
9.3
-
-
Cyanothece sp. ATCC 51142
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.33
6
10
-
Cyanothece sp. ATCC 51142