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Literature summary extracted from

  • Xie, B.B.; Bian, F.; Chen, X.L.; He, H.L.; Guo, J.; Gao, X.; Zeng, Y.X.; Chen, B.; Zhou, B.C.; Zhang, Y.Z.
    Cold adaptation of zinc metalloproteases in the thermolysin family from deep sea and arctic sea ice bacteria revealed by catalytic and structural properties and molecular dynamics: new insights into relationship between conformational flexibility and hydr (2009), J. Biol. Chem., 284, 9257-9269.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.25 expressed in Escherichia coli BL21(DE3) cells Pseudoalteromonas sp. SM495
3.4.24.25 expressed in Escherichia coli BL21(DE3) cells Pseudoalteromonas sp. SM9913

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.25 Pseudoalteromonas sp. SM495 B6E623
-
-
3.4.24.25 Pseudoalteromonas sp. SM9913 A1DRD5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.25 ammonium sulfate precipitation and DEAE-Sepharose column chromatography Pseudoalteromonas sp. SM495
3.4.24.25 ammonium sulfate precipitation and DEAE-Sepharose column chromatography Pseudoalteromonas sp. SM9913

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.25 3-(2-furylacryloyl)glycyl-L-leucine + H2O
-
 Pseudoalteromonas sp. SM495 ?
-
 ?
3.4.24.25 3-(2-furylacryloyl)glycyl-L-leucine + H2O
-
 Pseudoalteromonas sp. SM9913 ?
-
 ?
3.4.24.25 casein + H2O
-
 Pseudoalteromonas sp. SM495 ?
-
 ?
3.4.24.25 casein + H2O
-
 Pseudoalteromonas sp. SM9913 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.25 E495
-
 Pseudoalteromonas sp. SM495
3.4.24.25 MCP-02
-
 Pseudoalteromonas sp. SM9913

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.25 57
-
 using casein as substrate, at pH 7.5 Pseudoalteromonas sp. SM495
3.4.24.25 57
-
 using casein as substrate, at pH 8.0 Pseudoalteromonas sp. SM9913

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.25 40 70
-
 Pseudoalteromonas sp. SM495
3.4.24.25 40 70
-
 Pseudoalteromonas sp. SM9913

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.25 55 64 the melting temperature is at 64°C, the half-life at 55°C is 82 min, the half-life at 60°C is 20 min Pseudoalteromonas sp. SM495
3.4.24.25 55 64 the melting temperature is at 64°C, the half-life at 55°C is 90 min, the half-life at 60°C is 23 min Pseudoalteromonas sp. SM9913

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.25 7.5
-
 using casein as substrate, at 60°C Pseudoalteromonas sp. SM495
3.4.24.25 8
-
 using casein as substrate, at 60°C Pseudoalteromonas sp. SM9913

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.25 1.32
-
 3-(2-furylacryloyl)glycyl-L-leucine at 25°C, at pH 8.0 Pseudoalteromonas sp. SM9913
3.4.24.25 2.55
-
 3-(2-furylacryloyl)glycyl-L-leucine at 25°C, at pH 7,5 Pseudoalteromonas sp. SM495