BRENDA - Enzyme Database

Primary steps of the Na+-translocating NADH:ubiquinone oxidoreductase catalytic cycle resolved by the ultrafast freeze-quench approach

Bogachev, A.V.; Belevich, N.P.; Bertsova, Y.V.; Verkhovsky, M.I.; J. Biol. Chem. 284, 5533-5538 (2009)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.1.1
NAD+
competitive inhibitor
Vibrio harveyi
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Vibrio harveyi
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio harveyi
Na+-NQR reduction is not limited by the rate of NADH binding
NAD+ + ubiquinol + n Na+/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.1.1
Vibrio harveyi
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.2.1.1
-
Vibrio harveyi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Na+-NQR reduction is not limited by the rate of NADH binding
712407
Vibrio harveyi
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
-
Vibrio harveyi
7.2.1.1
FMN
two covalently bound FMNs
Vibrio harveyi
7.2.1.1
NADH
-
Vibrio harveyi
7.2.1.1
riboflavin
-
Vibrio harveyi
7.2.1.1
ubiquinone-8
-
Vibrio harveyi
7.2.1.1
[2Fe-2S]-center
-
Vibrio harveyi
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
7.2.1.1
11
-
NAD+
at 30C, 100 mM KCl, 20 mM HEPES/Tris (pH 7.5)
Vibrio harveyi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
-
Vibrio harveyi
7.2.1.1
FMN
two covalently bound FMNs
Vibrio harveyi
7.2.1.1
NADH
-
Vibrio harveyi
7.2.1.1
riboflavin
-
Vibrio harveyi
7.2.1.1
ubiquinone-8
-
Vibrio harveyi
7.2.1.1
[2Fe-2S]-center
-
Vibrio harveyi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.1.1
NAD+
competitive inhibitor
Vibrio harveyi
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
7.2.1.1
11
-
NAD+
at 30C, 100 mM KCl, 20 mM HEPES/Tris (pH 7.5)
Vibrio harveyi
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Vibrio harveyi
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio harveyi
Na+-NQR reduction is not limited by the rate of NADH binding
NAD+ + ubiquinol + n Na+/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
-
Vibrio harveyi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Na+-NQR reduction is not limited by the rate of NADH binding
712407
Vibrio harveyi
NAD+ + ubiquinol + n Na+/out
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
7.2.1.1
physiological function
Na+-NQR is a component of respiratory chain and generates a redox-driven transmembrane electrochemical Na+ potential
Vibrio harveyi
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.2.1.1
physiological function
Na+-NQR is a component of respiratory chain and generates a redox-driven transmembrane electrochemical Na+ potential
Vibrio harveyi